中国病理生理杂志Issue(10)：1879-1882,4.DOI:10.3969/j.issn.1000-4718.2014.10.026
Nodosin对HepG2细胞株的增殖抑制作用及对Bcl-2和Bax表达的影响
Inmbition of nodosin on cell proliferation and expression of Bcl-2 and Bax in HepG2 cells
摘要
Abstract
AIM:To investigate the effects of nodosin extracted from Chinese traditional medicine on the pro-liferation of HepG2 cells cultured in vitro and to detect the protein expression of Bcl-2 and Bax in HepG2 cells.METH-ODS:HepG2 cells were treated with different concentrations (1.25, 2.5, 5, 10 and 20 μmol/L) of nodosin for 24 h. The morphological changes of HepG2 cells were observed under inverted microscope.The inhibitory rates of HepG2 cell growth were detected by MTT assay.The apoptotic rates and the protein expression of Bcl-2 and Bax were analyzed by flow cytometry.RESULTS:Shrunken and suspended HepG2 cells increased with the increases in the concentrations of nodo-sin.The apoptotic rates and the expression of Bax increased with the increases in the doses of nodosin, while the expression of Bcl-2 decreased.CONCLUSION:Nodosin inhibits the growth of HepG2 cells in a dose-dependent manner.The inhibi-tion of HepG2 cell growth is induced by decreasing Bcl-2 and increasing Bax, thus promoting cell apoptosis.关键词
HepG2细胞/Nodosin/细胞凋亡Key words
HepG2 cells/Nodosin/Apoptosis分类
医药卫生引用本文复制引用
海广范,牛秉轩,李品品,郭兰青,崔泰震,李生莹..Nodosin对HepG2细胞株的增殖抑制作用及对Bcl-2和Bax表达的影响[J].中国病理生理杂志,2014,(10):1879-1882,4.基金项目
国家自然科学基金资助项目（No．81072552）；河南省科技攻关计划 （）
