中国病理生理杂志Issue(12)：2219-2225,7.DOI:10.3969/j.issn.1000-4718.2014.12.018
美沙拉嗪对DSS诱导小鼠溃疡性结肠炎模型Th1、Th17及Treg细胞亚群的影响
Effect of mesalazine on Th1, Th17 and Treg cells in mice with DSS-in-duced ulcerative colitis
摘要
Abstract
AIM:To investigate the effect of mesalazine treatment on regulation of Th1, Th17 and Treg cells in mice with dextran sulfate sodium ( DSS)-induced ulcerative colitis ( UC) .METHODS:The expression of IL-17, IFN-gam-ma and Foxp3 in the peripheral blood mononuclear cells ( PBMC) and intestinal mucosa lamina propria mononuclear cells ( LPMC) of DSS-induced UC mice was detected by flow cytometry analysis.The effect of mesalazine treatment on regulaiton of Th1, Th17 and Treg cells in the mice with DSS-induced ulcerative colitis was examined.RESULTS:The expression of IL-17, IFN-γand Foxp3 on CD4 +T cells were significantly higher in the PBMC of DSS-induced mice than those in control group.CD4+IFN-γ+T cells and CD4 +Foxp3 +T cells were higher in LPMC than those in control group, except CD4+IL-17 +T cells.Moreover, the Th1, Th17 and Treg cells were higher in DSS group than those in control group in LPMC.How-ever, only Tregs was higher in PBMC.Pre-treatment with mesalazine significantly decreased the number of Th17, Th1 and Treg cells of UC model mice both in PBMC and LPMC.CONCLUSION: The Th1, Th17 and Tregs cells in DSS-induced mice were significantly higher than those in control mice, suggesting that CD4 +T cell subsets play an important role in the pathogenesis of UC.Mesalazine may play a role in the treatment of UC by regulating the Th1, Th17 and Tregs cells.关键词
葡聚糖硫酸钠/溃疡性结肠炎/美沙拉嗪Key words
Dextran sulfate sodium/Colitis, ulcerative/Mesalazine分类
医药卫生引用本文复制引用
祝斌,张寒仙,曾今诚,安博然,徐军发,舒建昌..美沙拉嗪对DSS诱导小鼠溃疡性结肠炎模型Th1、Th17及Treg细胞亚群的影响[J].中国病理生理杂志,2014,(12):2219-2225,7.基金项目
国家自然科学基金资助项目（No．81273237；No．30972779）；广东省科技计划项目 （）
