中国病理生理杂志Issue(12)：2226-2231,6.DOI:10.3969/j.issn.1000-4718.2014.12.019
1α，25-二羟维生素D3对早期角膜移植小鼠Th17细胞的免疫调控作用
Effect of 1α, 25-dihydroxyvitamin D3 on T helper cell 17 and expression of related cytokines in penetrating keratoplasty in mice
摘要
Abstract
[ ABSTRACT] AIM:To evaluate the effects of 1α, 25-dihydroxyvitamin D3 on T helper cell 17 ( Th17 cells) and its related cytokines in a mouse model of corneal allograft transplantation.METHODS:C57BL/6 mice were transplanted with corneal grafts from BALB/c mice and treated intraperitoneally with 1.0μg 1α, 25-dihydroxyvitamin D3 or soybean oil every other day after operation.The transparency of the corneal grafts was evaluated for potential rejection signs by slit lamp biomicroscopy and histopathology.The expression levels of IL-17, RORγt and IFN-γin the spleen were measured by real-time PCR.Moreover, the protein expression of RORγt and IL-17 in the peripheral blood was analyzed by Western blotting. IL-17 and IFN-γin peripheral blood were measured by flow cytometry.RESULTS:1α, 25-dihydroxyvitamin D3 signifi-cantly inhibited the rejection of the corneal allograft and reduced the numbers of inflammatory infiltrates in the corneal graft. In the spleen, 1α, 25-dihydroxyvitamin D3 treatment reduced the expression levels of IL-17, RORγt and IFN-γ.In the pe-ripheral blood, 1α, 25-dihydroxyvitamin D3 treatment downregulated the expression levels of RORγt, IL-17 and IFN-γ. CONCLUSION:The effects of 1α, 25-dihydroxyvitamin D3 on suppressing corneal transplantation-induced allograft rejec-tion in mice are closely associated with its modulation on IL-17 and related cytokine RORγt.关键词
1α,25-二羟维生素D3/角膜移植术/辅助性T细胞17/白细胞介素17/维甲酸相关孤儿受体γtKey words
1α, 25-Dihydroxyvitamin D3/Keratoplasty/T helper cell 17/Interleukin-17/Retinoid-related orphan receptorγt分类
医药卫生引用本文复制引用
吴静,张瑾,侯光辉,崔裕波,王超,陈剑..1α，25-二羟维生素D3对早期角膜移植小鼠Th17细胞的免疫调控作用[J].中国病理生理杂志,2014,(12):2226-2231,6.基金项目
珠海市重点科技项目 （）
