中国病理生理杂志Issue(12)：2259-2261,3.DOI:10.3969/j.issn.1000-4718.2014.12.025
CD147对前列腺癌PC-3细胞自噬的影响
CD147 affects autophagy of prostate cancer PC-3 cells
摘要
Abstract
[ ABSTRACT] AIM: To study the autophagy of prostate cancer PC-3 cells induced by CD147 in vitro.ME-THODS:Themethod of amino acid starvation to induce autophagy was used.The expression of CD147 was detected by Western blotting.To study the functional effects of CD147 on autophagy in prostate cancer PC-3 cells, the down-regulation of CD147 expression was induced by the technique of RNAi.The conversion of autophagic marker protein LC3-I to LC3-II was determined by Western blotting.The cell death after starvation-induced autophagy was analyzed by trypan blue exclu-sion assay.RESULTS:The CD147 expression gradually increased in starvation-induced autophagy.The down-regulation of CD147 significantly increased the expression of autophagy-related protein LC3-II compared with control group.Mean-while, the cell death rates increased from (19.3 ±3.1)%and (22.3 ±3.5)%in control groups to (38.4 ±3.1)%in si-lencing the expression of CD147 in the PC-3 cells (P<0.05).CONCLUSION:CD147 inhibits starvation-induced auto-phgy and autophagy death in the prostate cancer PC-3 cells.关键词
抗原,CD147/PC-3细胞/自体吞噬Key words
Antigens,CD147/PC-3 cells/Autophagy分类
医药卫生引用本文复制引用
方芳,冯淳,姚杨,马潇潇,王立国..CD147对前列腺癌PC-3细胞自噬的影响[J].中国病理生理杂志,2014,(12):2259-2261,3.基金项目
国家自然科学基金资助项目（No．81202031）；吉林省教育厅“十二五”教育技术研究项目（吉教科合字2012第333号）；吉林省科技厅科技发展计划（No．20130101154JC）；吉林省自然科学基金资助项目（No．201015239）吉林省教育厅“十二五”科学技术研究重点项目 （）
