中国病理生理杂志Issue(1)：119-123,5.DOI:10.3969/j.issn.1000-4718.2015.01.023
PLCE1通过调控 p53抑制肺腺癌 A549细胞凋亡
PLCE1 suppresses apoptosis of lung adenocarcinoma cells via modulating p53 expression
摘要
Abstract
AIM:To investigate the role of phospholipase C epsilon 1 ( PLCE1 ) in modulating the apoptotic mechanism in lung adenocarcinoma A 549 cells.METHODS:PLCE1 inhibitor U-73122 was used to suppress the expres-sion of PLCE1.The expression of PLCE1 and p53 in A549 cells was evaluated by quantitative real-time PCR and Western blotting.Apoptosis was assessed by flow cytometry .RESULTS:A549 cells expressed high level of PLCE1 and low level of p53.Inhibition of PLCE1 markedly increased the expression of p 53, and increased the apoptosis of A 549 cells.CON-CLUSION:PLCE1 suppresses apoptosis of A549 cells via inhibiting the expression of p53.关键词
肺腺癌/磷脂酶Cε1/p53/细胞凋亡/甲基化/表观遗传学Key words
KEY WORDS] Lung adenocarcinoma/Phospholipase C epsilon 1/p53/Apoptosis/Methylation/Epigenomics分类
医药卫生引用本文复制引用
李昀,罗学平,林继宗,黄邵洪,安军,何锦园,张军航..PLCE1通过调控 p53抑制肺腺癌 A549细胞凋亡[J].中国病理生理杂志,2015,(1):119-123,5.基金项目
中山大学“985”工程项目 （）
