中国病理生理杂志Issue(1)：130-134,5.DOI:10.3969/j.issn.1000-4718.2015.01.025
促性腺激素释放激素类似物对人乳腺癌细胞的影响
Effect of gonadotropin-releasing hormone analogue on human breast cancer cell line in vitro
张晓娟 1董士中 1马艳庆 1杨鹿鸣1
作者信息
- 1. 平煤神马医疗集团总医院内分泌科，河南平顶山467000
- 折叠
摘要
Abstract
AIM:To investigate the effects of gonadotropin-releasing hormone ( GnRH) analogue on the growth of breast cancer cell lines MCF-7 and MDA-MB-231 in vitro and to explore the related mechanisms with PI 3K/Akt or ERK/MAPK pathways .METHODS: The proliferation of human breast cancer cell lines MCF-7 and MDA-MB-231 treatment with triptorelin was detected by MTT assay and the distribution of the cell cycle was determined by flow cytometry .The phosphorylation of the ERK 1/2 and Akt was evaluated by Western blotting .RESULTS:Triptorelin inhibited the prolifera-tion of MCF-7 cells at concentration of 10-5 mol/L after treated for 192 h or at concentration of 10 -4 mol/L after treated for 168 h and 192 h.Triptorelin inhibited the proliferation of MDA-MB-231 cells at concentration of 10 -4 mol/L after treated for 192 h (P<0.05).Treatment with triptorelin for 192 h at concentration of 10 -4 mol/L had no statistical significance effect on phosphorylation of ERK1/2 and Akt(P>0.05).CONCLUSION:Inhibitory effect of GnRH analogue triptorelin on human breast cancer cells is not just the connection with the down-regulation of pituitary hormone , but also a direct in-hibitory effect.The role may not be involved in the activation of ERK /MAPK and PI3K/Akt signaling pathways .关键词
MCF-7细胞/MDA-MB-231细胞/促性腺激素释放激素/细胞外信号调节蛋白激酶1/2/PI3K/Akt信号通路Key words
KEY WORDS] MCF-7 cells/MDA-MB-231 cells/Gonadotropin-releasing hormone/Extracellulalr signal-regula-ted kinase 1/2/PI3K/Akt signaling pathway分类
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张晓娟,董士中,马艳庆,杨鹿鸣..促性腺激素释放激素类似物对人乳腺癌细胞的影响[J].中国病理生理杂志,2015,(1):130-134,5.
