中国病理生理杂志Issue(2)：193-200,8.DOI:10.3969/j.issn.1000-4718.2015.02.001
限食8周对大鼠不同类型骨骼肌收缩功能及线粒体生物合成的影响
Effects of calorie restriction for 8 weeks on contractile function and mito-chondrial biosynthesis in different types of rat skeletal muscles
摘要
Abstract
AIM:To investigate the influence of calorie restriction ( CR) on contractility and mitochondrial bi-osynthesis in different types of rat skeletal muscles .METHODS:CR rat model was set up by feeding 60%normal food in-take of control rat every day for 8 weeks.Soleus (SOL) and extensor digitorum longus (EDL) were isolated under anesthe-sia.The twitch tension, titanic tension and fatigue resistance of SOL and EDL in response to electrical stimulation were measured to reflect the contractile function of the muscles .The copy number ratio of mitochondrial gene cytochrome C oxi-dase subunit I ( COX I) to nuclear gene β-actin was determined to evaluate the mitochondrial biosynthesis .ATP content was measured to mirror mitochondrial function .RESULTS:Compared with control group , CR for 8 weeks significantly in-creased twitch tension and titanic tension of both SOL and EDL , but only improved fatigue resistance in SOL .Markedly in-crease in ATP content in both skeletal muscles by CR intervention was observed , especially in SOL .Although CR activated AMP-activated protein kinase (AMPK) in both 2 muscles, up-regulation of mitochondrial biosynthesis and transcription of mitochondrial regulatory genes peroxisome proliferator-activated receptor γcoactivator 1α( PGC-1α) and nuclear respirato-ry factor ( NRF) was only observed in SOL .CONCLUSION:CR for 8 weeks enhanced the contractility of both rat SOL and EDL in response to electrical stimulation , especially in SOL composed of slow-twitch fibers.The mechanisms may be related to the activation of AMPK and the promotion of mitochondrial biosynthesis in SOL .关键词
限食/比目鱼肌/趾长伸肌/骨骼肌收缩/线粒体生物合成Key words
Calorie restriction/Soleus/Extensor digitorum longus/Skeletal muscle contractility/Mitochon-drial biosynthesis分类
医药卫生引用本文复制引用
邱霓,李聪,方伟进,何玉莲,韦雪梅,熊燕..限食8周对大鼠不同类型骨骼肌收缩功能及线粒体生物合成的影响[J].中国病理生理杂志,2015,(2):193-200,8.基金项目
国家自然科学基金资助项目（No．81170778）；广东省科技计划（No．2013B021800098）；广州市科技计划 （）
