中国病理生理杂志Issue(2)：239-244,6.DOI:10.3969/j.issn.1000-4718.2015.02.009
miR-708-5 p 通过抑制 TMEM88表达促进人骨髓间充质干细胞迁移
miR-708-5p accelerates migration of human mesenchymal stem cells by repressing TMEM88 expression
摘要
Abstract
AIM: To investigate the effect of microRNA-708-5p (miR-708-5p) on the migration of human mesenchymal stem cells (hMSCs).METHODS:The expression of miR-708-5p was determined by miRNA arrays and re-al-time PCR.By transfection of miR-708-5p mimic or inhibitor, the up-regulation or down-regulation of miR-708-5p ex-pression in hMSCs was evaluated .The cell scratch and Transwell tests were used to detect the migration capability of hM-SCs.The effects of transmembrane protein 88 (TMEM88), a miR-708-5p target gene, onβ-catenin expression and migra-tion of hMSCs were detected .RESULTS:The expression of miR-708-5p was down-regulated in the old hMSCs compared with the young hMSCs.Up-regulation of miR-708-5p resulted in increasing migration of hMSCs.Conversely, down-regula-tion of miR-708-5p resulted in decreasing cell migration .The expression of TMEM88 was up-regulated in the old hMSCs compared with the young hMSCs , while the expression of β-catenin was down-regulated.Directly repression of TMEM88 expression increased the β-catenin expression and migration of hMSCs .The regulation of miR-708-5p on hMSCs was atten-uated by inhibiting the expression of miR-708-5p and TMEM88 together.CONCLUSION:miR-708-5p increases β-cate-nin expression and Wnt/β-catenin activity by repressing TMEM 88, thus enhancing the migration of hMSCs .关键词
人骨髓间充质干细胞/MicroRNA-708-5p/年龄/细胞迁移Key words
Human mesenchymal stem cells/MicroRNA-708-5p/Age/Cell migration分类
医药卫生引用本文复制引用
黎佼,刘宁宁,胡明,曾波航,董珺,刘世明..miR-708-5 p 通过抑制 TMEM88表达促进人骨髓间充质干细胞迁移[J].中国病理生理杂志,2015,(2):239-244,6.基金项目
国家自然科学基金青年基金资助项目（No．81401156）；广州市医药卫生科技项目 （）
