中国病理生理杂志Issue(2)：256-260,5.DOI:10.3969/j.issn.1000-4718.2015.02.012
抑制肌球蛋白轻链激酶活性对内皮素-1诱导的大鼠肺动脉平滑肌细胞增殖及凋亡失衡的影响
Effects of inhibiting myosin light chain kinase on endothelin-1 induced proliferation and apoptosis of pulmonary arterial smooth muscle cells in rats
摘要
Abstract
AIM: To investigate the effect of inhibiting myosin light chain kinase ( MLCK) on endothelin-1 (ET-1) induced proliferation and apoptosis of rat pulmonary artery smooth muscle cells (PASMCs).METHODS: Rat PASMCs were cultured and stimulated with ET-1.The cells were randomly divided into control group , ET-1 group and ET-1+MLCK inhibitor group (ET-1+M).Western blotting, MTT assay, [3H]-TdR incorporation and flow cytometry were employed to test the expression of myosin light chain (MLC) and MLCK, cell proliferation, cell cycle and apoptotic rate of PASMCs ,respectively .The phosphorylation of MLC was determined by glycerol-PAGE coupled with Western blotting .RE-SULTS:Compared with control group , the protein expression of MLCK and MLC phosphorylation significantly enhanced af -ter ET-1 stimulation.ET-1 markedly induced the proliferation and decreased the percentage of apoptotic rate in the PASMCs.However, pretreatment with ML-7, a MLCK inhibitor, significantly reversed the above effects induced by ET-1. CONCLUSION:MLCK inhibitor effectively inhibits the ET-1-induced proliferation and the cell cycle progression .关键词
肌球蛋白轻链激酶/内皮素-1/肺动脉平滑肌细胞/细胞增殖/细胞凋亡Key words
Myosin light chain kinase/Endothelin-1/Pulmonary artery smooth muscle cells/Cell prolifera-tion/Apoptosis分类
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杨永曜,杨天和,蒋清安,杨龙,唐峰,谭洪文..抑制肌球蛋白轻链激酶活性对内皮素-1诱导的大鼠肺动脉平滑肌细胞增殖及凋亡失衡的影响[J].中国病理生理杂志,2015,(2):256-260,5.基金项目
贵州省科技攻关项目（黔科合SY字［2008］3065号）；贵州省优秀科技教育人才省长资金 （）
