中国病理生理杂志Issue(3)：440-446,7.DOI:10.3969/j.issn.1000-4718.2015.03.010
MicroRNA-1284在胃癌中的表达及其作用机制研究
Expression of microRNA-1284 in gastric cancer and underlying mecha-nism
摘要
Abstract
AIM:To evaluate the correlation between microRNA-1284 (miR-1284) and gastric cancer, and to investigate the underlying mechanism.METHODS: The expression of miR-1284 was examined by real-time PCR in 63 gastric cancer ( GC) tissue samples and 63 non-malignant adjacent tissue samples.The correlation between miR-1284 and the clinicopathological feature of GC was analyzed.Lentiviral vector containing miR-1284 was constructed and transfected into GC SGC-7901 cells.After transfection, the expression of miR-1284 was examined by real-time PCR.The cell activity was evaluated by CCK-8 assay.The cell cycle and apoptosis were determined by flow cytometry.The ability of cell migra-tion was measured by wound-healing assay.The potential target gene of miR-1284 was predicted by online bioinformatic softwares.The expression of JAG1 mRNA was examined by real-time PCR.The protein levels of JAG1, Notch1 and NF-κB were analyzed by Western blotting.RESULTS:Compared with non-malignant adjacent tissue samples, the results of real-time PCR showed significant downregulation of miR-1284 in 42 GC tissue samples ( P<0.05 ) .The expression level of miR-1284 was not significantly associated with age and gender of the patients, tumor size, TNM staging and lymph node metastases (P>0.05), but significantly associated with histologic grading (P<0.05).Compared with LV-NC-GFP group and control group, after transfection of miR-1284 in LV-miR-1284 group, the expression of miR-1284 was significantly in-creased (P<0.05), the percentages of apoptotic cells and the cells in G0/G1 phase were significantly increased (P<0.05), the cells activity and ability of migration were significantly decreased (P<0.05), and the expression of JAG1, Notch1 and NF-κB was significantly decreased (P<0.05).CONCLUSION:The inhibitory effect of miR-1284 on gastric cancer may be associated with the regulation of its targeting gene JAG1.关键词
微小RNA-1284/胃肿瘤/慢病毒载体/JAG1Key words
MicroRNA-1284/Stomach neoplasms/Lentiviral vectors/JAG1分类
医药卫生引用本文复制引用
韦尉元,曹稳珑,张笑石,詹泽栩,余晗,谢玉波,肖强..MicroRNA-1284在胃癌中的表达及其作用机制研究[J].中国病理生理杂志,2015,(3):440-446,7.基金项目
广西科学技术攻关项目 （）
