中国病理生理杂志Issue(3)：499-504,6.DOI:10.3969/j.issn.1000-4718.2015.03.020
扰乱昼夜节律对小鼠角膜创伤修复的影响
Disruption of circadian rhythms inhibits wound healing of corneal epithe-lium in mice
摘要
Abstract
[ ABSTRACT] AIM: To observe the effect of circadian rhythms on wound healing of mouse corneal epithelium. METHODS:The C57BL/6 male mice were used in the study.A part of corneal epithelium (2 mm in diameter) was struck off by a golf-like knife to form a round wound area.The dynamics of epithelial healing in the wound area were ob-served under microscope with fluorescein staining.In addition, with related antibodies and DAPI, the dynamic changes of the neutrophils, platelets and dividing cells were also investigated.RESULTS:The healing rates in LL group (12 h light/12 h light) and DD group (12 h dark/12 h dark) were obviously slower than that in LD group (12 h light/12 h dark), mainly showing delayed re-epithelialization, decreased epithelial cells, increased diameter of blood vessel, and delayed re-cruitment of neutrophils and platelets, but more cell number.CONCLUSION:Disruption of circadian rhythms significant-ly inhibits the wound healing of corneal epithelium, mainly through delaying the inflammation and re-epithelialization, but aggravating the inflammatory responses.关键词
角膜/昼夜节律/创伤修复/炎症反应Key words
Cornea/Circadian rhythms/Wound healing/Inflammation分类
医药卫生引用本文复制引用
刘鹏,刘俊,薛芸霞,宋方,王寒晴,傅婷,夏潮涌,李志杰..扰乱昼夜节律对小鼠角膜创伤修复的影响[J].中国病理生理杂志,2015,(3):499-504,6.基金项目
国家自然科学基金资助项目 （）
