中国病理生理杂志Issue(3)：539-542,4.DOI:10.3969/j.issn.1000-4718.2015.03.027
钙激活氯通道 ANO1在小鼠心肌细胞的表达及其功能鉴定
Expression and identification of ANO1 in mouse cardiomyocytes
摘要
Abstract
AIM:To explore the expression of anoctamin 1 (ANO1), one of calcium-activated chloride chan-nels ( CaCCs) , in mouse cardiomyocytes and its functional properties.METHODS:The cardiomyocytes from the myocar-dial tissues of C57BL/6 mice were isolated with enzyme and purified by the differential adherent method.The cells were stained with monoclonal anti-sarcomeric actin and Cy3 to evaluate the purity of the myocardial cells.RT-PCR was used to detect the mRNA expression of ANO1 in the mouse cardiomyocytes.The protein expression of ANO1 in the mouse cardio-myocytes was determined by Western blotting analysis.The fluorescence quenching kinetics experiment was used to identify the ion transport properties of ANO1 in the mouse cardiomyocytes.RESULTS: The results of RT-PCR confirmed that ANO1 was expressed in freshly isolated myocardial cells.The results of Western blotting clearly demonstrated the protein expression of ANO1 in primarily cultured myocardial cells.Fluorescence quenching kinetics experiment on freshly isolated single myocardial cell revealed a pronounced outward rectifying property of the ANO1.The functional properties were simi-lar to the classic CaCCs.CONCLUSION:ANO1 expression was identified in the mouse myocardial cells.The function of CaCCs was generated by ANO1, suggesting that ANO1 is the molecular basis of CaCCs.关键词
Anoctamin 1/钙激活氯离子通道/心肌细胞Key words
Anoctamin 1/Calcium-activated chloride channels/Cardiomyocytes分类
医药卫生引用本文复制引用
侯毅鞠,许会静,张雲乔,扈昕虹,郝峰..钙激活氯通道 ANO1在小鼠心肌细胞的表达及其功能鉴定[J].中国病理生理杂志,2015,(3):539-542,4.基金项目
国家自然科学基金资助项目（No．81202031）；吉林省教育厅基金资助课题（No．2013351）；2014年吉林省大学生创新创业训练计划；2013年吉林省大学生创新创业训练计划；吉林医药学院大学生科研基金资助课题 （）
