中国病理生理杂志Issue(4)：597-602,6.DOI:10.3969/j.issn.1000-4718.2015.04.004
IL-8促进 A549细胞迁移过程中对线粒体融合与分裂基因表达的影响
Effect of IL-8 on mitochondrial fusion and fission gene expression in hu-man lung adenocarcinoma cell line A549 during cell migration
摘要
Abstract
[ ABSTRACT] AIM:To investigate the changes of migration of lung adenocarcinoma cells promoted by IL-8 and the inner and outer mitochondrial membrane dynamic changes during this process.METHODS:Human lung adenocarci-noma cell line A549 was divided into control group and IL-8 group.Cell migration was analyzed by scratch detection and Transwell assay.The secretion of endogenous IL-8 was detected by ELISA.The protein levels of mitochondrial cytochrome C ( Cyt C) and mitochondrial outer membrane protein Tom20 was detected by Western blot.The mRNA expression of mito-chondrial fusion genes Mfn1, Mfn2 and OPA1 and fission genes Fis1, Drp1 and MTP18 was detected by RT-PCR.The morphological changes of mitochondria were observed by MitoTracker Red CMXRos dye staining and confocal microscopy. RESULTS:The migratory rate of A549 cells and endogenous secretion of IL-8 in A549 cells were higher than those in SPC-A-1 cells.The migratory rate of A549 cells was improved by IL-8 in a time-dependent manner.Compared with control group, the Tom20 protein expression was increased ( P<0.05 ) , and the Cyt C protein expression was decreased ( P<0.05).The expression of mitochondrial outer membrane fusion genes Mfn1 and Mfn2 was increased (P<0.05), and the expression of mitochondrial inner membrane fusion gene OPA1 was decreased (P<0.05).The expression of fission genes Drp1 and MTP18 were decreased (P<0.05), while the expression of Fis1 was no change (P>0.05).Under confocal microscope, the punctate aggregates in the mitochondria of the A549 cells treated with IL-8 were observed.CONCLU-SION:The migratory rate of A549 cells is increased by IL-8, which is related to the changes of mitochondrial fusion genes and the fission genes.关键词
肺腺癌/细胞迁移/线粒体/IL-8Key words
Lung adenocarcinoma/Cell migration/Mitochondria/IL-8分类
医药卫生引用本文复制引用
张钰,高宇飞,苏静,于春艳,贺宜春,孙连坤..IL-8促进 A549细胞迁移过程中对线粒体融合与分裂基因表达的影响[J].中国病理生理杂志,2015,(4):597-602,6.基金项目
国家自然科学基金资助项目（ No．81272876；No．81372696）；中国博士后科学基金资助项目（ No．2013M541314）；教育部留学回国人员科研启动基金资助项目 （）
