中国病理生理杂志Issue(4)：620-624,5.DOI:10.3969/j.issn.1000-4718.2015.04.008
DEC1基因过表达对人食管癌 ECA109细胞增殖及侵袭能力的影响
Effect of DEC1 gene over-expression on proliferation and invasion abili-ties of human esophageal cancer ECA109 cells
杨纯平 1王华川 1温剑虎1
作者信息
- 1. 重庆医科大学附属第一医院胸心外科，重庆400016
- 折叠
摘要
Abstract
[ ABSTRACT] AIM:To investigate the effect of DEC1 gene over-expression on the proliferation and invasion abili-ties of human esophageal cancer ECA109 cells.METHODS: ECA109 cells were transfected with plasmid pcDNA3.1 (-)/DEC1 (DEC1 group) or pcDNA3.1 (-) (vector group).The mRNA and protein levels of DEC1, cyclin D1 and MMP-9 were evaluated by real-time PCR and Western blot, respectively.The effects of DEC1 over-expression on the prolif-eration and invasion abilities of the ECA109 cells were evaluated by CCK-8 assay, colony formation assay and Transwell test respectively.RESULTS:The DEC1 expression level in ECA109 cells in DEC1 group was significantly higher than that in vector group (P<0.01), but the levels of MMP9 and cyclin D1 expression were opposite (P<0.01).However, both the proliferation and invasion abilities of ECA109 cells in DEC1 groups decreased significantly as compared with those in vector group (P<0.05).CONCLUSION:The over-expression of DEC1 significantly inhibits the proliferation and invasion of ECA109 cells, which may be involved in the expression of cyclin D1 and MMP9.关键词
DEC1基因/食管癌/ECA109细胞/细胞增殖/细胞侵袭Key words
DEC1 gene/Esophageal cancer/ECA109 cells/Cell proliferation/Cell invasion分类
医药卫生引用本文复制引用
杨纯平,王华川,温剑虎..DEC1基因过表达对人食管癌 ECA109细胞增殖及侵袭能力的影响[J].中国病理生理杂志,2015,(4):620-624,5.
