中国病理生理杂志Issue(4)：659-663,5.DOI:10.3969/j.issn.1000-4718.2015.04.015
CX3CR1参与雷公藤内酯对MPP＋帕金森病大鼠多巴胺能神经元的保护作用
CX3CR1 mediates the neuroprotective effect of triptolide on 1-methyl-4-phenylpyridinium-induced hemiparkinson rats
摘要
Abstract
[ ABSTRACT] AIM:To investigate the effect of triptolide on the inhibition of microglial activation in 1-methyl-4-phenyl pyridinium ( MPP+)-induced hemiparkinson disease rats.METHODS:The rat model of Parkinson disease was es-tablished by intranigral injection of MPP +.The rats were randomly divided into sham group, MPP+group, triptolide group and vehicle group.The survival of dopaminergic neurons was detected by the immunofluorescence of tyrosine hydroxylase ( TH) in the substantia nigra ( SN) .The activation of microglia was determined by immunofluorescence of OX-42 ( micro-glia marker) in the SN.The expression of chemokine receptor CX3CR1 in SN was measured by Western blotting.RE-SULTS:Intranigral injection of MPP+increased the fluorescence intensity of the microglial marker, and promoted DA neu-ron degenerative death.Immunohistological analysis showed that the OX-42 density was decreased (P<0.01) and tyrosine hydroxylase (TH) positive neurons were increased in the triptolide group (P<0.01).The expression of CX3CR1 was lower in triptolide group than that in model group (P<0.05).CONCLUSION:Triptolide may improve PA neurons func-tion in MPP+-induced rats through inhibiting CX3CR1 expression and microglial activation.关键词
帕金森病/小胶质细胞/雷公藤内酯/多巴胺能神经元Key words
Parkinson’ s disease/Microglia/Triptolide/Dopaminergic neuron分类
医药卫生引用本文复制引用
周子懿,高俊鹏,向军,陈依萍,蔡业峰,罗恩丽,蔡定芳..CX3CR1参与雷公藤内酯对MPP＋帕金森病大鼠多巴胺能神经元的保护作用[J].中国病理生理杂志,2015,(4):659-663,5.基金项目
国家自然科学基金资助项目 （）
