中国病理生理杂志Issue(5)：802-807,6.DOI:10.3969/j.issn.1000-4718.2015.05.006
吡格列酮对尿毒症 ApoE－／－小鼠调节性和效应T 细胞平衡的影响
Effect of pioglitazone on balance of regulatory and effector T cells of ure-mic apolipoprotein E knockout mice in vitro
摘要
Abstract
AIM:To investigate the effects of pioglitazone on the quantity and function-related factors of regu-latory and effector T cells ( Treg and Teff ) of uremic apolipoprotein E knockout mice in vitro with or without the stimulation of atherosclerotic plaque-specific antigen oxidized low-density lipoprotein ( oxLDL) .METHODS:Uremic apolipoprotein E knockout mouse model was established by 2-step surgical procedure.After intervention with different concentrations ( 2μmol/L and 20μmol/L) of pioglitazone and PPARγantagonist GW9662 (5μmol/L) on splenocytes of uremic mice for 12 h in the presence or absence of oxLDL (2 mg/L), the levels of CD4 +CD25 +Foxp3 +Treg and IFNγ+CD4 +Teff were de-termined by flow cytometry.The mRNA expressions of Foxp3 and IFNγwas detected by real-time fluorescent quantitative PCR.RESULTS:In vitro, oxLDL induced a Treg/Teff imbalance in splenocytes from the uremic mice.Pioglitazone up-regulated the level of Treg and mRNA expression of Foxp3 in the presence of oxLDL, which was not antagonized by GW9662.Meanwhile, pioglitazone downregulated the level of Teff and mRNA expression of IFNγin the presence or ab-sence of oxLDL, which was reversed by GW9662.CONCLUSION:oxLDL induces a Treg/Teff imbalance in uremic apo-lipoprotein E knockout mice.Pioglitazone modulates the Treg/Teff imbalance probably through PPARγ-independent and-dependent mechanisms.关键词
吡格列酮/过氧化物酶体增殖物激活受体γ/尿毒症/调节性T细胞/效应T细胞Key words
Pioglitazone/Peroxisome proliferator-activated receptor γ/Uremia/Regulatory T cells/Effector T cells分类
医药卫生引用本文复制引用
申燕,肖嫣,王丽君,赵艳,田雨灵,梁潇,尹爱萍..吡格列酮对尿毒症 ApoE－／－小鼠调节性和效应T 细胞平衡的影响[J].中国病理生理杂志,2015,(5):802-807,6.基金项目
国家自然科学基金资助项目（No．81200541）；中央高校基本科研业务费专项资金 （）
