中国病理生理杂志Issue(5)：834-838,5.DOI:10.3969/j.issn.1000-4718.2015.05.012
脊髓小胶质细胞 P2 X4受体在 rrTNF 诱导的病理性疼痛中的作用
Effects of P2X4 receptor in spinal microglia on rrTNF-induced pathologi-cal pain
摘要
Abstract
AIM:To investigate the effects of P2X4 receptor on peri-sciatic administration of recombinant rat TNF-α(rrTNF)-induced mechanical allodynia.METHODS:Male Sprague-Dawley rats (180~200 g) were used in the experiments.The levels of P2X4 receptor on day 3, day 7 and day 14 after peri-sciatic administration of rrTNF were exam-ined by Western blot, and the location of P2X4 receptor in the spinal dorsal horn was observed by double immunofluores-cence staining.The changes of 50%paw-withdrawal thresholds of the rat were detected by behavioral test, and the level of TNF-αin the spinal dorsal horn was also examined by Western blot when TNP-ATP was intrathecally injected before the ad-ministration of rrTNF.RESULTS:Compared with control group, the expression of P2X4 receptor in the spinal dorsal horn on the ipsilateral side significantly increased on day 3, day 7 and day 14 (P<0.01) after rrTNF (100 ng/L) administra-tion.P2X4 receptor was co-localized only with microglia, but not with neurons or astrocytes.Intrathecal injection of TNP-ATP before rrTNF administration prevented mechanical allodynia induced by rrTNF and inhibited the upregulation of TNF-αin the spinal dorsal horn.CONCLUSION:P2X4 receptors in microglia may be involved in rrTNF-induced mechanical allodynia by the upregulation of TNF-αin the spinal dorsal horn.关键词
P2X4 受体/肿瘤坏死因子α/病理性疼痛/脊髓背角/小胶质细胞Key words
P2X4 receptor/Tumor necrosis factor-alpha/Pathological pain/Spinal dorsal horn/Microglia分类
医药卫生引用本文复制引用
宫庆娟,汪红华,梁颖,卢振和,陈金生,黄乔东,越宇..脊髓小胶质细胞 P2 X4受体在 rrTNF 诱导的病理性疼痛中的作用[J].中国病理生理杂志,2015,(5):834-838,5.基金项目
国家自然科学基金资助项目（No．31100805）；广州市珠江科技新星专项资助项目（No．2012J2200036）；广州市卫生局西医一般引导项目（No．20141A011081）；国家临床重点建设专科项目 （）
