中国病理生理杂志Issue(6)：1036-1041,6.DOI:10.3969/j.issn.1000-4718.2015.06.013
iPSC－MSCs 对 CoCl2诱导的 PC12细胞损伤时线粒体功能的影响
Effects of iPSC-MSCs on mitochondria of PC12 cells injured by CoCl2
摘要
Abstract
[ ABSTRACT] AIM:To investigate the effects of induced pluripotent stem cells-derived mesenchymal stem cells ( iPSC-MSCs) on cobalt chloride ( CoCl2 )-induced injuries of PC12 cells and its possible mechanism.METHODS:PC12 cells were exposed to CoCl2 to set up a chemical-induced cellular injury model and were cocultured with iPSC-MSCs.The cell viability was tested by CCK-8 assay.The apoptosis was measured by flow cytometry using Annexin V/PI staining.The mitochondrial membrane potential (MMP) was analyzed by flow cytometry using JC-1 staining.Immunofluorescence was employed to observe mitochondrial transfer from iPSC-MSCs to PC12 cells.RESULTS: Apoptosis of PC12 cells was in-creased and MMP of PC12 cells was decreased after exposed to CoCl2 at concentration of 400μmol/L for 24 h.Coculture of PC12 cells with iPSC-MSCs reduced the apoptosis and recovered the MMP of the PC12 cells.Tunneling nanotubes were formed between iPSC-MSCs and PC12 cells, through which the iPSC-MSCs transferred the mitochondria to the PC12 cells. CONCLUSION:iPSC-MSCs protect PC12 cells from CoCl2-induced injuries, which may be associated with the mitochon-drial transfer from iPSC-MSCs to PC12 cells.关键词
氯化钴/PC12细胞/细胞凋亡/诱导性多能干细胞/线粒体Key words
KEY WORDS] Cobalt chloride/PC12 cells/Apoptosis/Induced pluripotent stem cells/Mitochondria引用本文复制引用
杨焰,李慧,孙占朋,胡春林,荆小莉,魏红艳,廖晓星,李欣..iPSC－MSCs 对 CoCl2诱导的 PC12细胞损伤时线粒体功能的影响[J].中国病理生理杂志,2015,(6):1036-1041,6.基金项目
国家自然科学基金资助项目（ No．81272062； No．81471832； No．81372022）；广东省自然科学基金资助项目（No．S2013010016799）；广东省科技计划（No．2012B031800286）；中山大学青年教师重点培育项目 （）
