中国病理生理杂志Issue(7)：1172-1177,6.DOI:10.3969/j.issn.1000-4718.2015.07.004
MADPH 氧化酶抑制剂对晚期氧化蛋白产物诱导内皮细胞高通透性的影响及机制
Protective effect of diphenyleneiodonium, a NADPH oxidase inhibitor, on hyperpermeability of endothelial cells exposed to AOPP-HSA in vitro
摘要
Abstract
[ ABSTRACT ] AIM: To investigate the effect of advanced oxidation protein product-human serum albumin ( AOPP-HSA) at different concentrations on the permeability of human umbilical vein endothelial cell ( HUVEC) monolayer and the protective effect of NADPH oxidase inhibitor diphenyleneiodonium ( DPI ) against AOPP-HSA exposure. METHODS: Cultured HUVECs were exposed to 200 mg/L HSA (control) or AOPP-HSA (50, 100 and 200 mg/L).The permeability of the endothelial monolayer was assessed by measuring CMFDA-labeled THP-1 cells across the endothelial cells.The cultured HUVECs were treated with HSA (200 mg/L), AOPP-HSA (200 mg/L), or AOPP-HSA (200 mg/L)+DPI (100 μmol/L), and the activation of NADPH oxidase, endothelial monolayer permeability and cytoskeleton rear-rangement were evaluated.RESULTS: AOPP-HSA increased the permeability of the endothelial cell monolayer, and AOPP-HSA at 200 mg/L significantly increased the phosphorylation level of NADPH oxidase in the cells.Treatment with 100 μmol/L DPI obviously attenuated AOPP-HSA-induced NADPH oxidase activation, the increase in the permeability of the cell monolayer and the cytoskeleton rearrangement.CONCLUSION: AOPP-HSA increases the hyperpermeability of HUVEC monolayer via the phosphorylation of NADPH oxidase, and the NADPH oxidase inhibitor DPI reverses such effects.关键词
NADPH氧化酶/晚期氧化蛋白产物/内皮细胞/氧化应激/细胞通透性Key words
NADPH oxidase/Advanced oxidation protein products/Endothelial cells/Oxidative stress/Per-meability分类
医药卫生引用本文复制引用
张瑛,夏学颖,王春筱,王晓红,邹和群..MADPH 氧化酶抑制剂对晚期氧化蛋白产物诱导内皮细胞高通透性的影响及机制[J].中国病理生理杂志,2015,(7):1172-1177,6.基金项目
广东省医学科研基金资助项目（No．B2012244）；南方医科大学青年科技人员培育项目（No．201201547）；南方医科大学第三附属医院院长基金资助项目 （）
