中国草食动物科学Issue(5)：22-25,4.DOI:10.3969/j.issn.2095-3887.2014.05.006
云南半细毛羊毛囊干细胞无血清培养体系的建立
Establishment of Serum Free Culture of Hair Follicle Stem Cell in Yunnan Semi-fine Wool Sheep
摘要
Abstract
This experiment was aimed to study the separation, culture and identification methods of hair follicle stem cells(HF-SCs):for the development of further research on cell differentiation. Method:The tissue piece culture method was used to separate and culture HFSCs. Then the cultured cells were identified by immunohistochemistry.Result:The established HFSCs exhibited adherent growth type, small volume, high nucleus/cytoplasm ratio, high refractivity and transparent cell appearance. Immunoflu-orescence assay indicated that Yunnan semi-fine wool sheep HFSCs expressed K14, not expressed CD271. These results further proved that the cultured cells were indeed HFSCs.Conclusion:A serum free culture system of Yunnan semi-fine wool sheep HF-SCs was established using the tissue piece culture method. This study is very important for investigation of the regulation mecha-nism of development of wool follicle and the characterstics of proliferation and differentiation of HFSCs.关键词
云南半细毛羊/毛囊干细胞/分离培养Key words
Yunnan semi-fine wool sheep/HFSCs/separation and culture分类
农业科技引用本文复制引用
吕春荣,权国波,欧阳依娜,沈伟,洪琼花..云南半细毛羊毛囊干细胞无血清培养体系的建立[J].中国草食动物科学,2014,(5):22-25,4.基金项目
国家现代农业产业技术体系建设（CARS-40）；云南省应用基础研究青年项目 （）
