中国当代儿科杂志Issue(10)：1045-1050,6.DOI:10.7499/j.issn.1008-8830.2014.10.018
多巴胺受体调节对新生大鼠缺氧缺血性脑损伤后焦虑样行为的影响
Impact of dopamine receptor modulation on reduced anxiety-like behavior in neonatal rats after hypoxic-ischemic brain damage
摘要
Abstract
Objective To observe the long-term changes in anxiety-like behavior and tyrosine hydroxylase (TH) expression in the substantia nigra (SN) after hypoxic-ischemic brain damage (HIBD) in a neonatal rat model and to further explore the relationship between dopamine (DA) level and long-term anxiety-like behavior using the DA receptor (DAR) antagonist. Methods Seven-day-old (P7) neonatal Sprague-Dawley (SD) rats were randomized into normal control, sham-operated, HIBD and HIBD+DAR antagonist groups. HIBD model was prepared by ligating the right common carotid artery and 8%hypoxia exposure. The rats in the sham-operated group were sham-operated and were not subjected to right common carotid artery ligation and hypoxia exposure. The DAR antagonist was injected intraperitoneally before and after inducing HIBD. The same amount of normal saline was given to the other three groups as a control. Anxiety-like behavior was evaluated by elevated plus maze test, and TH expression in the SN was measured by immunohistochemistry on P14, P21, and P28. Results On P21 and P28, the time spent in the open arms and the percentage of open arms entries in the HIBD group were signiifcantly increased compared with those in the normal control, sham-operated and HIBD+DAR antagonist groups (P<0.05);in addition, the HIBD+DAR antagonist group showed a signiifcantly longer time spent in the open arms than the normal control group (P<0.05). On P14, P21, and P28, TH expression in the HIBD and HIBD+DAR antagonist groups was signiifcantly lower than that in the normal control and sham-operated groups, and TH level in the HIBD group was signiifcantly lower than that in the HIBD+DAR antagonist group (P<0.05). Conclusions DAR antagonist allows the restoration of anxiety-like behavior and alleviates the damage to dopaminergic neurons in SD rats after HIBD.关键词
多巴胺受体/缺氧缺血/焦虑样行为/酪氨酸羟化酶/大鼠Key words
Dopamine receptor/Hypoxia-ischemia/Anxiety-like behavior/Tyrosine hydroxylase/Rats引用本文复制引用
陶惠康,汤琴,戴津津,李媛媛,黑明燕..多巴胺受体调节对新生大鼠缺氧缺血性脑损伤后焦虑样行为的影响[J].中国当代儿科杂志,2014,(10):1045-1050,6.基金项目
中南大学中央高校基本科研业务费专项资金（2013zzts348）；中南大学湘雅三医院125人才基金课题。 （）
