中国当代儿科杂志Issue(11)：1172-1176,5.DOI:10.7499/j.issn.1008-8830.2014.11.022
动态监测婴儿肠道16S rDNA宏基因组5例报告
Dynamic changes of intestinal 16S rDNA metagenome in 5 infants
摘要
Abstract
Objective To investigate the dynamic changes of intestinal 16S rDNA metagenome in healthy infants. Methods Seventeen fecal samples were collected at ages of 3 days, 1 month, 6 months and 1 year in 5 infants. Total bacterial DNAs were extracted and submitted high throughout sequencing on the V6 viable region of 16S rDNA. Tags and Operational Taxonomic Units (OTU) were then obtained and analysis of taxonomy, abundance and alpha diversity were performed. Results In total 2 190.66 Mbp raw data in 17 samples were produced. The OTU numbers ranged from 36 to 308. The dominate phylum included Proteobacteria, Firmicutes and Bacteroidetes and Actinobacteria. The bacterial families>1%increased from only 2-4 per sample on day 3 to 7 at 1 or 6 months, 10 at 12 months. The average npShannon and Simpson index on day 3, at 1 month, 6 months and 1 year were 1.117, 1.460, 2.088, 2.50 and 0.443, 0.408, 0.229, 0.143 respectively. Conclusions Infants’ intestines harbor abounding bacterial genomes. Distinct individual differences exist in infants in terms of intestinal bacterial abundance and composition. The abundance and diversity of gut bacteria increase over time.关键词
宏基因组/测序/肠道菌群/婴儿Key words
Metagenome/Sequencing/Intestinal microbiota/Infant引用本文复制引用
马丽亚,张敏,王辉林,陈睿,黄艳,梁小琴,卢光进..动态监测婴儿肠道16S rDNA宏基因组5例报告[J].中国当代儿科杂志,2014,(11):1172-1176,5.基金项目
深圳市科技计划项目（201102030）。 （）
