中国当代儿科杂志Issue(12)：1271-1274,4.DOI:10.7499/j.issn.1008-8830.2014.12.017
大鼠大脑皮层星形胶质细胞体外培养改良方法的研究
A modiifed culture method for astrocytes from rat cortical tissuein vitro
摘要
Abstract
ObjectiveTo evaluate the efifciency of a modiifed culture method for rat cerebral cortical astrocytes in vitro.MethodsThe astrocytes derived from the cerebral cortex of 3-day-old Sprague-Dawley rats were ifrst puriifed as described previously, then the cells were replanted at a low density. The culture lfask was changed after 1 hour and substratum was replaced after 24 hours. Cells were syncretized to a monolayer, followed by cell passage. After three passages the cells were cultured in DMEM medium containing 10% fetal serum for a long period.The derivation of the cells was identiifed by immunolfuorescent staining with anti-GFAP polyclonal antibodies.ResultsA variety of morphologically distinct astrocytes with many long processes and small cell bodies were obtained. Finally an astrocytic network occurred through cellular process connections. The immunolfuorescent staining demonstrated the percentage of GFAP-positive cells was above 98%.ConclusionsThe modiifed culture method for astrocytes from rat cerebral tissue is reliable, with a high purity. The cultured astrocytes have a similar morphological development to thosein vivo.关键词
星形胶质细胞/细胞培养/神经胶质原纤维酸性蛋白/大鼠Key words
Astrocyte/Cell culture/Glial ifbrillary acidic protein/Rats引用本文复制引用
郭慧,毛萌,俞丹,周晖,童煜..大鼠大脑皮层星形胶质细胞体外培养改良方法的研究[J].中国当代儿科杂志,2014,(12):1271-1274,4.基金项目
国家自然科学基金 （30772434）。 （）
