中国当代儿科杂志Issue(4)：400-404,5.DOI:10.7499/j.issn.1008-8830.2015.04.022
缺氧缺血海马组织中自噬相关蛋白Beclin-1和LC3的表达变化以及雷帕霉素对其表达的影响
Changes in expression of autophagy-related proteins, Beclin-1 and LC3, and effects of rapamycin on their expression in hypoxic-ischemic hippocampus
摘要
Abstract
ObjectiveTo observe changes in the expression of autophagy-related proteins, Beclin-1 and LC3, in the hippocampal tissue of neonatal rats with hypoxic-ischemic brain damage (HIBD) at different time points, and to investigate the effect of rapamycin (Ra) on the expression of the above two proteins.MethodsA total of 108 7-day-old Sprague-Dawley rats were randomly divided into sham, HIBD, and Ra groups (n=36 each). The HIBD model was established using the modiifed Rice method. For sham rats, only the left common carotid artery was separated without ligation or hypoxic treatment. For Ra-treated rats, 0.5 mg/kg Ra was administered by an intraperitoneal injection 1 hour before model establishment. The rats were anesthetized and sacriifced to collect brain tissues at 0, 6, 12, 24, 48, and 72 hours after model establishment. Changes in the expression of Beclin-1 and LC3 proteins in rat hippocampus were examined by Western blot.ResultsThe expression level of Beclin-1 in HIBD rats began to increase at 0 hour, peaked at 24 hours, and then declined thereafter, similar as those of Beclin-1 and LC3-II in Ra-treated rats. The expression level of LC3-II in HIBD rats began to increase at 0 hour, peaked at 12 hours, and then declined thereafter. At all time points, both Beclin-1 and LC3-II expression levels were significantly higher in HIBD and Ra-treated rats than in sham rats (P<0.05); except LC3-II at 12 hours, Beclin-1 and LC3-II expression levels were signiifcantly higher in Ra-treated rats than in HIBD rats (P<0.05).ConclusionsHypoxia-ischemia activates autophagy in rat hippocampal cells, while Ra enhances the expression process of autophagy.关键词
海马/缺氧缺血/雷帕霉素/Beclin-1/LC3/大鼠Key words
Hippocampus/Hypoxia-ischemia/Rapamycin/Beclin-1/LC3/Rats引用本文复制引用
李荣虎,于论,缪珀,丁欣,孙斌,冯星..缺氧缺血海马组织中自噬相关蛋白Beclin-1和LC3的表达变化以及雷帕霉素对其表达的影响[J].中国当代儿科杂志,2015,(4):400-404,5.基金项目
国家自然科学基金（81271378）；苏州市科技发展计划项目（SYS201441）。 （）
