中国当代儿科杂志Issue(7)：736-740,5.DOI:10.7499/j.issn.1008-8830.2015.07.019
脐血单个核细胞移植联合高压氧治疗对新生大鼠缺氧缺血性脑损伤的影响
Effects of umbilical cord mononuclear cells transplantation combined with hyperbaric oxygen therapy on hypoxic-ischemic brain damage in neonatal rats
摘要
Abstract
ObjectiveTo study the effects of umbilical cord monoculcear cells (UCBMC) transplantation combined with hyperbaric oxygen (HBO) therapy on the long-term behaviors and histology in neonatal rats after hypoxic-ischemic brain damage (HIBD).MethodsSeven-day-old Sprague-Dawley rats were randomly assigned to four groups: normal control (CON), HIBD, UCBMC and UCBMC+HBO. HIBD was induced according to the Rice-Vannucci method. The rats in the UCBMC+HBO group were treated with HBO 3 hours after HIBD, followed by UCBMC transplantation 24 hours after HIBD. IL-1β and TNF-α protein levels were examined by Western blot analysis in the 4 groups. T-maze test and radial arm maze test were used to detect the long-term learning memory capability. Nissl staining was used to examine the histological changes of the hippocampal CA1 region.ResultsTwenty-four hours after transplantation, IL-1β and TNF-α protein levels in the UCBMC+HBO group were signiifcantly reduced compared with the HIBD (P<0.01) and UCBMC groups (P<0.05). The study and memory capabilities were impaired, and the number of the pyramidal cells in the hippocampal CA1 region was reduced in the HIBD group. The study and memory capabilities were greatly improved and the number of pyramidal cells increased signiifcantly in the UCBMC+HBO group compared with the UCBMC and HIBD groups (P<0.05).ConclusionsUCBMC transplantation combined with HBO therapy could reduce the expression of IL-1β and TNF-α protein, improve long-term behaviors and alleviate brain damages in the hypoxic ischemic neonatal rats.关键词
脐血单个核细胞/缺氧缺血性脑损伤/高压氧/新生大鼠Key words
Umbilical cord blood mononuclear cell/Hypoxic-ischemic brain damage/Hyperbaric oxygen/Neonatal rat引用本文复制引用
马泽,闫少珍,王晓莉,陈伟,曹向昱,董鹏,赵岩松..脐血单个核细胞移植联合高压氧治疗对新生大鼠缺氧缺血性脑损伤的影响[J].中国当代儿科杂志,2015,(7):736-740,5.基金项目
国家自然科学基金项目（81000268）；山东省自然科学基金项目（ZR2014JL049）；山东省医药卫生科技发展计划项目（2013WS0295）。 （）
