中国稻米Issue(3)：30-35,6.DOI:10.3969/j.issn.1006-8082.2014.03.006
云南三种野生稻遗传多样性和系统进化研究
Study on Genetic Diversity and Phylogeny Evolution of Three Wild Rice Species in Yunnan
摘要
Abstract
The genetic diversity in three wild rice species and bamboo, cultivated rice and O. longistaminata were assessed, and com-pared by ISSR method to determine their phylogenetic relationships, discuss the evolution direction of three kinds of Yunnan wild rice. The results indicated that a total of 464 alleles were detected among all the materials by 33 strips of ISSR primers, 14.1 polymorphic bands were amplified of the average of each primer. Rich genetic diversity existed in the three wild rice species at the aggregate level. The number of polymorphic loci(Np), the percentage of polymorphic loci(p), the effective number of alleles(Na), observed number of alleles(Ne), Nei 's genetic diversity index(H) and Shannon index(I) were 357, 76.74%, 1.7694±0.4217, 1.3391±0.3637, 0.2037± 0.1851 and 0.3180±0.2532. The coefficient of gene differentiation(Gst) was 0.0968 among the three wild rice species while the gene flow (Nm) was 0.4627, which shown that the gene exchanged at low level among three wild rice species and the genetic divergence was relatively high among rice varieties. UPGMA cluster analysis revealed the evolutionary process of three wild rice, O. meyeriana differentiats first, following by the O. Officinalis, O. rufipogon differentiats at the latest.关键词
野生稻/ISSR标记/亲缘关系/遗传多样性/遗传进化Key words
wild rice/ISSR markers/phylogenetic relationship/genetic diversity/phylogeny evolution分类
农业科技引用本文复制引用
陈玲,程在全,殷富有,李维蛟,李定琴,张敦宇,钟巧芳,陈越,柯学,黄兴奇..云南三种野生稻遗传多样性和系统进化研究[J].中国稻米,2014,(3):30-35,6.基金项目
云南省科技计划项目（2012CH010）；中科院国家重点实验室开放课题（2009-PCCE-KF-03）；国家自然基金项目（31160067）；云南省高端人才引进（2010CI120）；农业部行业专项 （）
