中国动物检疫Issue(8)：65-69,79,6.
猪瘟病毒C株杂交瘤细胞的构建及单克隆抗体的制备与鉴定
Preparation and Identiifcation of Monoclonal Antibodies against Classical Swine Fever Virus C Strain
摘要
Abstract
Four monoclonal antibodies designated as 1C9,1B11,3C8 and 3G9 against classical swine fever virus C strain(CSFV C strain) were generated by immunization of BALB/C mice with puriifed CSFV C strain. The titers of ascetic fluids of the four McAbs were up to 104~106 in indirect ELISA. The McAbs were only positive against CSFV, and negative against Newcastle disease virus of pigeon,porcine reproductive and respiratory syndrome virus,trans-missible gastroenteritis virus and egg drop syndrome virus. The preparation of the McAbs provided a basis to establish rapid diagnostic method of CSFV.关键词
猪瘟C株/杂交瘤细胞/单克隆抗体/鉴定Key words
CSFV C strain/hybridoma/monoclonal antibody/identiifcation分类
农业科技引用本文复制引用
杨明,陈伯祥,郭慧琳,李杰,豆林涛..猪瘟病毒C株杂交瘤细胞的构建及单克隆抗体的制备与鉴定[J].中国动物检疫,2014,(8):65-69,79,6.基金项目
甘肃省农业生物技术研究与应用开发项目 （）
