中国动物检疫Issue(9)：64-68,5.
鸭肝炎病毒的分离与鉴定
Isolation and Identiifcation of Duck Hepatitis Virus
徐保娟 1郭伟伟 1宫晓 1李陆梅 1宋新宇 1范根成1
作者信息
- 1. 青岛易邦生物工程有限公司，山东青岛 266032
- 折叠
摘要
Abstract
22 strains of duck hepatitis virus(DHV) were isolated from sick or died ducks with typical symptoms of duck hepatitis in Shandong,Henan,Zhejiang,Jiangsu,Guangxi,Shanxi,Jiangxi,Guangdong,Fujian and Lia-oning Provinces from 1999 to 2008. Serum neutralization test were conducted using duck hepatitis virus I type(DHV-1) antiserum and the new DHV(N-DHV) antiserum with these 22 strains. The VP1 genes of the isolated strains were amplified using RT-PCR and then sequenced. Amino acid sequences of VP1 were then deduced and analyzed. The results showed that there were two serotypes of DHV in China. Among the 22 strains,12 strains were in DHV-1 and 10 strains in N-DHV. The serotyping result based on the homology of VP1 gene sequences was identical to the result based on the antigenicity serotyping. Amino acid sequence homology was 95% within 12 DHV-1 strains and 98% within 10 N-DHV strains respectively. The amino acid homology of VP1 gene was 72~77% between DHV-1 and N-DHV strains. The less variation of VP1 gene in the same serotype strains suggested that the antigenicity of DHV was stable.关键词
鸭肝炎病毒/VP1基因/序列分析/血清型/分离/鉴定Key words
duck hepatitis virus/VP1 gene/sequence analysis/serotype/isolation/identification分类
农业科技引用本文复制引用
徐保娟,郭伟伟,宫晓,李陆梅,宋新宇,范根成..鸭肝炎病毒的分离与鉴定[J].中国动物检疫,2014,(9):64-68,5.
