中国动物检疫Issue(10)：66-69,4.
水貂源肺炎雷伯菌的分离鉴定及致病性测定
Isolation,Identiifcation and Virulence Determination of Klebsiella pneumoniae from mink
王剑 1韩耀东 1杨广1
作者信息
- 1. 山东省邹平县畜牧兽医局，山东邹平 256200
- 折叠
摘要
Abstract
To identify the main bacterial pathogens causing mink deaths in a mink farm. [Method] Two dominant bacterial isolates got from the dead minks submitted were subjected to culture and morphological observation,biochemical tests,drug susceptibility test,pathogenicity test and 16S-23S rRNA ISR sequencing. [Result] Results revealed that 16S-23S rRNA ISR gene of the two isolates shared 99.9%homology with K.pneumoniae reference strain. Drug sensitive test indicated that the bacterial isolates were highly sensible to sulbactam and ceftriaxone. Pathogenicity tests showed that the isolates could cause high morbidity or mortality in healthy mice and its median lethal dose was 4.9×106cfu/mL and 3.1×106cfu/mL respectively. [Conclusion] The two isolates belonged to Klebsiella peneumoniae and were the main pathogen causing mink deaths.关键词
水貂/肺炎克雷伯菌/分离鉴定/致病性Key words
Mink/Klebsiella pneumoniae/Isolation and Identification/Virulence分类
农业科技引用本文复制引用
王剑,韩耀东,杨广..水貂源肺炎雷伯菌的分离鉴定及致病性测定[J].中国动物检疫,2014,(10):66-69,4.
