中国动物检疫Issue(11)：97-100,4.
ELISA法检测非洲猪瘟抗体实验条件的优化
Optimizing the Conditions of an Indirect ELISA for Detection of African Swine Fever Virus Antibody
摘要
Abstract
In the study,the effects of antigen coating concentration,enzyme labeled antibody and chromogenic sub-strate in an ELISA with inactivated African swine fever virus as coating antigen were analyzed for detection of ASFV antibody. In order to obtain the optimum reaction conditions,different coating antigen concentrations,2 type enzyme-labeled antibody and 2 type chromogenic substrates were used in the ELISA to detect 2 positive serum samples and 1 negative serum sample. The result showed that the optimum reaction conditions were:the antigen coating concentration was 15μg/mL;Protein A-HRP was used as labeled antibody,and OPD as chromogenic substrate and the P/N value of 2 positive serum samples were 4.920 and 7.259.关键词
ELISA/非洲猪瘟抗体/试验验条件/优化Key words
ELISA/African swine fever virus antibody/test condition/optimization分类
农业科技引用本文复制引用
张永强,任伟杰,吴晓东,王筱真,李金明,王志亮..ELISA法检测非洲猪瘟抗体实验条件的优化[J].中国动物检疫,2014,(11):97-100,4.基金项目
公益性行业（农业）科研专项经费 （）
