中国动物检疫Issue(2)：57-62,6.
猪繁殖与呼吸综合征病毒美洲型RT-LAMP检测方法建立
Development of an RT-LAMP Assay for Detection of American Type Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)
摘要
Abstract
Two pairs of primers targeting six regions of American-type porcine reproductive and respiratory syndrome virus ORF6 gene,and RT-LAMP assay was established for the rapid detection of porcine reproductive and respiratory virus with high sensitivity and specificity. It could be finished in about 2 hours. The sensitivity of the assay was similar to PRRSV fluorescent RT-PCR,and higher than the normal RT-PCR. The inactivated porcine reproductive and respiratory syndrome virus culture was serially diluted in 10-folds,and tested with PRRSV fluorescent RT-PCR,RT-LAMP assay and normal RT-PCR. The result showed that the fluorescence RT-PCR detection limit was up to 10-7,the normal RT-PCR detection limit was 10-5,and the RT-LAMP detection limit was 10-8,indicating RT-LAMP assay was very sensitive. The assay could be used for rapid detection of the American-type porcine reproductive and respiratory syndrome virus in various pig tissue samples.关键词
猪繁殖与呼吸综合征病毒/美洲型/RT-LAMPKey words
porcine reproductive and respiratory syndrome/American-type virus/RT-LAMP分类
农业科技引用本文复制引用
蒲静,肖春芳,汪琳,尹羿,柏亚铎,任彤,张伟,高志强,乌日古玛拉,时培蛟..猪繁殖与呼吸综合征病毒美洲型RT-LAMP检测方法建立[J].中国动物检疫,2015,(2):57-62,6.基金项目
国家质检总局科技计划项目（2013IK027）资助 （）
