中国动物检疫Issue(3)：66-69,4.
犬弓首蛔虫线粒体cox1基因的克隆及序列分析
Analysis of Sequence Variation in Mitochondrial cox1 Gene of Toxocara canis Isolates in China
梁轩 1刘毅1
作者信息
- 1. 湖南农业大学动物医学院，湖南长沙 410128
- 折叠
摘要
Abstract
The objective of the study was to analyze sequence variation in the cytochrome c oxidase subunit 1(cox1) gene among Toxocara canis isolates from Hunan province and to study its phylogenetic relationship with other round-worms using the cox1 gene sequencing. The partial cox1(pcox1)were ampliifed from individual T. canis samples, and pcox1 sequences were aligned using the Mafft 7.122. Maximum likelihood(ML)trees were constructed using the software PhyML 3.1. The lengths of all pcox1 sequences were 394bp. Sequence variations in pcox1 sequences within T. canis were 0~2.5%which was signiifcantly lower than inter-species differences(8.2%~11.6%). Phylogenetic analyses showed that all T. canis isolates were clustered in the same clade. It was concluded that pcox1 sequence could be used as genetic marker for population genetic studies of T. canis,as well as genetic marker for the differentiation and identiifca-tion of different roundworms. The study provided foundation for further studies on molecular genetics and diagnosis of T. canis.关键词
犬弓首蛔虫/线粒体DNA/cox1基因/种系发育关系Key words
Toxocara canis/mitochondrial DNA/cox1 gene/phylogenetic relationship分类
农业科技引用本文复制引用
梁轩,刘毅..犬弓首蛔虫线粒体cox1基因的克隆及序列分析[J].中国动物检疫,2015,(3):66-69,4.
