中国动物检疫Issue(4)：14-17,31,5.
对一株超强马立克氏病毒株的检测和研究
Detection and Analysis of a Very Virulent Marek’s Disease Virus Strain
摘要
Abstract
In this study,a very virulent Marek's disease virus(vvMDV) strain was isolated from the infected chicken farm. The meq gene of the isolated strain was amplified by PCR and sequenced. It was found that nucleotide homology of the isolated strain with the very virulent virus strain SD2012-1 was as high as 99%. Compared with the vaccine strain CVI988/Rispens,3 bases(CAC) were inserted at site 575-577 of meq gene of the isolate and SD2012-1. Virus challenge and vaccination showed that the incidence in the unimmunized group was 80.0%;the incidence in conventional-dose HVT group and 10×conventional-dose HVT group were 73.0% and 76.6% respectively. HVT couldn't provide effective protection. The incidence of conventional-dose CVI988 group and the 10×conventional-dose group CVI988 was both 30.0%. The incidence of conventional-dose group HVT + CVI988 and 10× conventional-dose HVT + CVI988 group were 26.6% and 26.7% respectively. The incidence of CVI988 vaccine group and HVT + CVI988 combined vaccine group was lower than those in the HVT vaccine group. In short,the virulence enhancement due to wild strain variation had broken through the existing vaccine immune protection,thus bringing serious losses to the poultry industry.关键词
超强马立克氏病毒/meq致瘤基因/疫苗/发病率Key words
very virulent Marek's disease virus strain/meq gene/vaccine/incidence分类
农业科技引用本文复制引用
张康,单虎,龚振华,林祥超,郭光礼,杜翔,王丽萍,于建敏,李金平,侯广宇..对一株超强马立克氏病毒株的检测和研究[J].中国动物检疫,2015,(4):14-17,31,5.基金项目
本项目由农业部科技计划项目和青岛市科技计划项目支持 （）
