中国动物检疫Issue(6):75-78,4.
鱼类神经坏死病毒纳米探针的制备与特征分析
Production and Characterization of Silver Nanoprobe for Detection of Fish Nervous Necrosis Virus
摘要
Abstract
A nanoprobe(Ag-NNV)was prepared and characterized for detecting fish nervous necrosis virus. The morphology and spectroscopic properties of Ag-NNV nanoprobe were evaluated by transmission electron microscopy, automatic microplate reader and surface-enhanced Raman microscope. The results showed that the Ag-NNV nanoprobe was sphere-shaped with average diameter of 90 nm and dispersed well in the solution. The number of hairpin probe at-tached on a silver nanoparticle was estimated to be about 100 and the coverage density was 0.56pmol/cm2. With easy preparation,the Ag-NNV nanoprobe can be used for quick detection of fish nervous necrosis virus by surface enhanced Raman scattering.关键词
纳米探针/神经坏死病毒/表面增强拉曼散射Key words
nanoprobe/nervous necrosis virus/surface-enhanced Raman scattering(SERS)分类
农业科技引用本文复制引用
粟子丹,史成银,刘江春,王胜强..鱼类神经坏死病毒纳米探针的制备与特征分析[J].中国动物检疫,2015,(6):75-78,4.基金项目
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