中国肺癌杂志Issue(2)：92-97,6.DOI:10.3779/j.issn.1009-3419.2015.02.08
EFEMP1通过下调MMP-7表达抑制肺癌细胞生长和侵袭
EFEMP1 Suppresses Growth and Invasion of Lung Cancer Cells by Downregulating Matrix Metalloproteinase-7 Expression
摘要
Abstract
Background and objective EFEMP1, a member of ifbulin family proteins, is a very important extracel-lular matrix protein which is involved in cell metabolism and its role in tumor occurrence and progression is still poorly under-stood. hTe aim of this study is to investigate the functional effect and mechanism of EFEMP1 in lung cancer cell growth and invasion. Methods EFEMP1 expression in lung cancer cells was determined by Western blot. hTe promoter methylation status of EFEMP1 was detected by methylation-speciifc PCR (MSP). Atfer transfection of control or EFEMP1 vector in lung cancer cells, the ability of colony formation and invasion was detected by colony formation experiment and matrigel invasion method. Western blot and real-time PCR were used to detect matrix metalloproteinase-7 (MMP-7) expression. Luciferase assay was used to detect expression of MMP-7 reporter construct transfected with or without EFEMP1 in lung cancer cells. Results Western blot result showed EFEMP1 expression was downregulated in lung cancer cells. hTe promoter region of EFEMP1 was methyl-ated in A549 and H1299 and atfer treatment with 5-aza-2’-deoxycytidine, the EFEMP1 expression was upregulated. hTe growth and invasion of A549 and H1299 were all signiifcantly suppressed by transfecting with EFEMP1 and the MMP-7 expression was dowanregulated by EFEMP1 as well. Expression activity of MMP-7 reporter construct was decreased by cotransfecting with EFEMP1. Conclusion Collectively, these results suggest that EFEMP1 functions as a suppressor of lung cancer growth and inva-sion. Epigenetic silencing of EFEMP1 promotes lung cancer invasion and metastasis by activating MMP-7 expression.关键词
EFEMP1/MMP-7/肺肿瘤/细胞侵袭Key words
EFEMP1/MMP-7/Lung neoplasms/Invasion引用本文复制引用
郎媛媛,孟洁,宋晓萌,陈小军..EFEMP1通过下调MMP-7表达抑制肺癌细胞生长和侵袭[J].中国肺癌杂志,2015,(2):92-97,6.基金项目
本研究受天津市自然科学基金项目（No.14JCQNJC12100）、天津市教委科学基金项目（No.2012ZD01）、天津医科大学科学基金项目（No.2010ky07）资助 （）
