中国肺癌杂志Issue(5)：266-271,6.DOI:10.3779/j.issn.1009-3419.2015.05.03
胸腔内注射重组人血管内皮抑素对裸鼠恶性胸腔积液的治疗作用
Therapeutical Effects of Pleural Injecting Recombinant Human Endostain to Malignant Pleural Effusion Nude Mice Model
摘要
Abstract
Background and objective hTe prognosis of malignant pleural effusion (MPE) was poor, injecting anti-angiogenesis agents in pleural cavity might be to reducing the volume of pleural effusion. hTe aim of this study is to investigate the therapeutical effect of pleural injection of recombinant human endostain, cisplatin and recombinant human endostain combined with cisplatin to MPE nude mice. Methods MPE model was built by intrpleural injection of Lewis lung cancer cells (LCC) into BALB/c nude mice. Intrpleural injection of recombinant human endostain (E), cisplatin (P) and recombinant hu-man endostain combined with cisplatin (EP) was performed, MPE volume was measured, immunohistochemistry of CD31 was carried out to calculate micro vessel density (MVD), angiogenesis and apoptosis gene expression was detected. Results MPE volume was reduced by intrapleiral injection of recombinant human endostain and recombinant human endostain com-bined with cisplatin, MPE volume was positive correlated with MVD. Vescular epidermal growth factor-α(VEGF-α) expression reduced simultaneously with expression of hypoxia induced factor-1 (HIF1-α) elevated at the same time. Conclusion MPE model could be made by intrapleural injection of LLC. Intrapleural injection of recombinant human endostain could reduce MPE volume of nude mice. hTe potential molecular mechanism of the therapeutical effects of intapleural injection of recombi-ant endostatin might be related to the downregulation of VEGF-αexpression and neovascularization.关键词
肺肿瘤/胸腔积液/重组人血管内皮抑素Key words
Lung neoplasms/Pleural effusion/Recombinant human endostain引用本文复制引用
周明,李敏,杨华平,胡成平..胸腔内注射重组人血管内皮抑素对裸鼠恶性胸腔积液的治疗作用[J].中国肺癌杂志,2015,(5):266-271,6.基金项目
本研究受十二五国家科技支撑计划课题（No.2013BI09B09）资助 （）
