中国感染控制杂志Issue(9)：524-529,6.DOI:10.3969/j.issn.1671-9638.2014.09.003
临床分离大肠埃希菌耐药性分析及I型整合子研究
Antimicrobial resistance analysis on clinically isolated Escherichia coli and detection of class I integrons
摘要
Abstract
Objective To study antimicrobial resistance of clinically isolated Escherichia coli (E.coli),the preva-lence of integrons in E.coli,and relation of integron with antimicrobial resistance of E.coli.Methods E.coli isola-ted from three hospitals of Guangdong Province from 2010 to 2012 were collected,and antimicrobial susceptibility testing was performed by Kirby-Bauer method;integrons were detected by polymerase chain reaction (PCR),and gene cassette was analyzed by sequencing.Results A total of 156 E.coli isolates were collected,antimicrobial sus-ceptibility testing showed that resistance rate of E.coli to most penicillins,cephalosporins,fluoroquinolones,amin-oglycosides and sulfonamides were over 50%;the resistance rate to antimicrobials < 10% included cefoperazone/sulbactam(0),imipenem(3.85%),cefotetan(4.35%),ertapenem(7.69%)and piperacillin /tazobactam (8.97%);The positive rate of class I integron was 57.69%(90/156);the positive rate of class I integron in multidrug-resist-ant and non-multidrug-resistant E.coli was 66.00% (66/100)and 64.71 % (22/34)respectively,the difference was not statistically different (P >0.05),but compared with sensitive E.coli (9.09%,2/22),the difference was statisti-cally different (P<0.01 ).There were nine types of integron-drug resistant gene cassettes in the variable regions, most of which contained aadA and dfrA.Conclusion Antimicrobial resistance of E.coli is serious;high incidence of class I integrons are widely found in E.coli,and is closely related with drug resistance of E.coli,class I integrons mainly mediated aminoglycosides,sulfonamides and beta-lactams resistance.关键词
整合子/大肠埃希菌/耐药机制/多重耐药/抗药性,微生物/抗菌药物/合理用药Key words
integron/Escherichia coli/drug-resistance mechanism/multidrug-resistance/drug resistance,mi-crobial/antimicrobial agent/rational drug use分类
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黄小荣,刘配辰,蔡瑞昭,陈晓燕,黄浩泉,周俊宜..临床分离大肠埃希菌耐药性分析及I型整合子研究[J].中国感染控制杂志,2014,(9):524-529,6.基金项目
中山大学实验室开放基金 （）
