中国骨伤Issue(6)：461-465,5.DOI:10.3969/j.issn.1003-0034.2014.06.005
河蚌提取物葡聚糖对软骨细胞Wnt通路的调控作用研究
Regulating effect of anodonta glucan HBP-A on chondrocytes through Wnt pathway
摘要
Abstract
Objective:To investigate regulation function of anodonta glucan HBP-A on chondrocytes through Wnt path-way in vitro. Methods:Rat chondrocytes were cultured and differentiated induced with IL-1β (10 ng/ml) in vitro. Chondro-cytes were divided into five groups:IL-1βgroup,IL-1β+IWP-2 (5μM,Wnt pathway inhibitor) group,IL-1β+HBP-A (0.3 mg/ml) group and IL-1β+IWP-2+HBP-A group. Wnt-3a,β-catenin (24 h,48 h,72 h) and MMP-13(72 h) genes expres-sion were detected by Rt-PCR,whileβ-catenin,MMP-13,Sox-9 and coll-Ⅱ (48 h) protein expression were measured by Western-blot. Results:After induction of IL-1β,gene expression of Wnt-3a,β-catenin and MMP-13 were increased,so were the protein expression ofβ-catenin and MMP-13. In contrast,protein expression of Sox-9 and Coll-Ⅱwere declined. Follow-ing addition of HBP-A,Wnt-3a,β-catenin and MMP-13 were shown as induction of IL-1β,but protein expression of Sox-9 and Coll-Ⅱwere upgraded . Combining HBP-A with IWP-2 led to the lowest level in Wnt-3a ,β-catenin gene andβ-catenin protein expression and highest expression of Sox-9 protein. Conclusion:HBP-A could not only delay the differentiation of chondrocytes through downgrading the signal expression of Wnt/β-catenin,but also adjust the expression of Wnt-3a,β-catenin and Sox-9 when combinated with the Wnt inhibitor.关键词
组织提取物/软骨细胞/葡聚糖类/基因表达调控Key words
Tissue extracts/Chondrocytes/Glucans/Gene expression regulation引用本文复制引用
韦宋谱,丁道芳,王学宗,庞坚,郑昱新,徐勤光,曹月龙,詹红生..河蚌提取物葡聚糖对软骨细胞Wnt通路的调控作用研究[J].中国骨伤,2014,(6):461-465,5.基金项目
国家自然科学基金（编号81072830）；上海市教委创新项目（编号11YZ64）；上海中医药事业发展三年行动计划项目（编号ZYSNXD-CC-YJXYY）Fund programProvided by National Natural Science Foundation of China （）
