中国骨伤Issue(9)：766-771,6.DOI:10.3969/j.issn.1003-0034.2014.09.013
低强度脉冲超声对兔膝骨关节炎软骨细胞外基质的影响及机制
Effects and mechanism of low intensity pulsed ultrasound on extracellular matrix in rabbit knee osteoarthritis
摘要
Abstract
Objective:To observe the effects of low intensity pulsed ultrasound (LIPUS) on repairing extracellular matrix in rabbit knee osteoarthritis and analyze its mechanism. Methods:Sixty adult female rabbits with an average weight of (2.0± 0.2) kg,were divided randomly into two groups (experimental group and control group,30 rabbits in each group). All rabbits were replicated in right knees by Hulth method for knee osteoarthritis model. Two weeks after operation ,the rabbits in experi-mental group were treated with LIPUS,and the ultrasonic frequency was (800±5%)KHz and the maximum intensities of spa-tially averaged and time averaged(SATA) was (50±10%) mw/cm2,for 1 time a day and every time 20 min,while the rabbits in control group were treated with sham LIPUS ,the same operation with experimental group but without energy output. At the 2,4,8 weeks after treatment,10 rabbits in each group were randomly killed for each time. The general changes of cartilage and its histopathological changes by HE staining were observed;the expression of collagen typeⅡ,proteoglycan,MMP-3,7,13 in cartilage were analyzed by immunohistochemical and RT-PCR technique;and the expression of NO in cartilage was analyzed by nitrate reduction method. Results:On the same observed time point,the damage degree of cartilage in experimental group was slighter than that of control group(P<0.01),the expression of MMP-3,7,13 and NO in cartilage in experimental group was lower than that of control group (P<0.01) while collagen typeⅡand proteoglycan was higher than that of control group (P<0.01). Conclusion:Low intensity pulsed ultrasound can repair the damaged cartilage by reducing the expression of MMP-3,7,13,inhibiting the secretion of NO and promoting the synthesis of collagen typeⅡand proteoglycan in cartilage.关键词
低强度脉冲超声/骨关节炎,膝/软骨细胞/细胞外基质Key words
Low intensity pulsed ultrasound/Osteoarthritis,knee/Chondrocytes/Extracellular matrix引用本文复制引用
徐守宇,张丽梅,姚新苗,周国庆,李霞,何邦剑,陈小刚..低强度脉冲超声对兔膝骨关节炎软骨细胞外基质的影响及机制[J].中国骨伤,2014,(9):766-771,6.基金项目
浙江省医药卫生科技计划项目（编号2012KYA137）；国家中医药管理局重点学科建设经费资助项目［编号国中医药人教发（2012）32号］Fund programThe Medical Health Science and Technology Projects in Zhejiang Province （）
