局解手术学杂志Issue(3)：244-246,3.DOI:10.11659/jjssx.1672-5042.201403009
bFGF和TGF-β对原发性骨关节炎软骨间充质祖细胞增殖调控作用
Regulatory effect of bFGF and TGF-βfor the proliferation of mesenchymal progenitor cells derived from prima-ry osteoarthritis cartilage
摘要
Abstract
Objective To observe the regulatory effect of bFGF and TGF-β1 for the proliferation of mesenchymal progenitor cells ( MPCs) derived from primary osteoarthritis cartilage, and to provide theoretical evidence in preventing and curing primary OA. Methods Different concentrations of bFGF and TGF-β1 ( alone or combined) were used to treat primary OA cartilage and their effects on proliferation of MPCs were tested by MTT method. Results Either bFGF (10. 0~50. 0 ng/mL) or TGF-β1 (0. 1 ~1. 0 ng/mL) alone can significantly promote the proliferation of MPCs derived from primary OA cartilage (P<0. 05). But with their increased concentration,the proliferation rate was of no significant changes (P>0. 05). The combination of 10. 0 ng/mL bFGF and 1. 0 ng/mL TGF-β1 significantly increased the prolifer-ation of MPCs from primary OA (P<0. 05). Conclusion Both bFGF and TGF-β11 play important roles in the proliferation of MPCs in primary OA cartilage,and they can increase the proliferation in different degree with different concentrations. There must be feasible methods of gene technology to promote cell proliferation and differentiation of MPCs for repairing articular car-tilage injury.关键词
骨关节炎/间充质祖细胞/bFGF/TGF-βKey words
osteoarthritis/mesenchymal progenitor cells/bFGF/TGF-β分类
医药卫生引用本文复制引用
刘军,王洪伟,陈语,于海龙,王琪,杨会峰,马骏雄,项良碧..bFGF和TGF-β对原发性骨关节炎软骨间充质祖细胞增殖调控作用[J].局解手术学杂志,2014,(3):244-246,3.基金项目
国家自然科学基金面上项目（30672200,C030313) （）
