局解手术学杂志Issue(3)：253-255,256,4.DOI:10.11659/jjssx.01E015061
罗格列酮对高糖环境下人脐静脉内皮细胞作用的研究
Effects of rosiglitazone on activation of human umbilical vein endothelial cells in high glucose environment
摘要
Abstract
Objective To investigate the effects of rosiglitazone on proangiogenesis function of human umbilical vein endothelial cells ( HUVEC) in high glucose environment. Methods HUVECs were cultured in high glucose environment and stimulated by rosiglitazone. MTT, cell scratch test and Transwell assay were used to detect HUVEC proliferation and migration. The concentration of VEGF, SDF-1 was also detected in the supernatant. Results Rosiglitazone could effectively promote HUVEC proliferation and migration. The concentration of VEGF and SDF-1 in rosiglitazone stimulated supernatant was higher than that in high glucose group. The inhibition of AKT signal could block the promotion of rosiglitazone on the HUVEC proliferation, migration and secretion. Conclusion Rosiglitazone could significantly promote HUVEC secretion, proliferation and migration in high glucose environment. AKT signal played an important role in this process.关键词
罗格列酮/人脐静脉内皮细胞/高糖环境/活化Key words
rosiglitazone/HUVEC/high glucose environment/activation分类
医药卫生引用本文复制引用
杨明璨,熊鸿燕,林辉..罗格列酮对高糖环境下人脐静脉内皮细胞作用的研究[J].局解手术学杂志,2015,(3):253-255,256,4.基金项目
国家自然科学基金面上项目(31470046) （31470046）
