局解手术学杂志Issue(4)：370-372,373,4.DOI:10.11659/jjssx.01E015043
MSC复合生物材料促进缺血性糖尿病溃疡愈合的研究
Research of MSC-seeded biomaterials promoting ischemic diabetic ulcers repair
摘要
Abstract
Objective To observe the effect of mesenchymal stem cells ( MSC) in treating ischemic diabetic ulcers, and to explore its clinical perspectives. Methods Prepared electro-spinning biomaterials and cultured MSC to study effect of MSC composite biomaterials in vitro by scanning electron microscope,MTT array and influence of MSC conditioned medium on endothelial cells. The use of 5 to 8 week old male C57BL/6J mice were prepared into diabetic mice,femoral artery ligation in the proximal thigh,in dorsal skin full-thickness wounds caused by the diameter 5 mm. Then the effect of MSC composite biomaterials on ischemic diabetic ulcers was determined. Results This study found that the MSC grow well on electro-spinning biomaterials. Cells foot extension and connections between cells were observed by scanning electron microscope. Stimulated by high glucose,growth and proliferation of MSC has a stronger ability on biomaterials. MSC condi-tioned medium on biomaterials increased human umbilical vein endothelial cells proliferation ability. Conclusion MSC composite biomateri-als can effectively improve the treatment effect of MSC on ischemic diabetic ulcers. The study indicated stem cells composite biomaterials have great potential and application prospect in the treatment of ischemic diabetic ulcers healing.关键词
间质干细胞(MSC)/生物材料/缺血性糖尿病溃疡/血管生成Key words
mesenchymal stem cells ( MSC)/biomaterials/ischemic diabetic ulcers/angiogenesis分类
医药卫生引用本文复制引用
柏力,杨明璨,谭菊,孙建森..MSC复合生物材料促进缺血性糖尿病溃疡愈合的研究[J].局解手术学杂志,2015,(4):370-372,373,4.基金项目
国家自然科学基金资助项目(31200731) （31200731）
