中国康复理论与实践Issue(9)：827-830,4.DOI:10.3969/j.issn.1006-9771.2014.09.009
葡萄籽原花青素对脑缺血再灌注大鼠氧化应激与学习记忆的影响
Effects of Grape Seed Proanthocyanidin Extract on Peroxidation and Ability of Learning and Memory after Cerebral Ischemia/re-perfusion Injury in Rats
王珠 1赵雅宁 1李建民1
作者信息
- 1. 河北联合大学护理与康复学院，河北唐山市063000
- 折叠
摘要
Abstract
Objective To investigate the effect of grape seed proanthocyanidin extract (GSPE) on learning and memory ability after cere-bral ischemia/reperfusion injury in rats and its mechanism. Methods 72 healthy male Sprague-Dawley rats were divided into sham group (n=18), model group (n=18) and GSPE groups (20 mg/kg, 200 mg/kg, n=18 for each group). The GSPE groups were administered GSPE orally for 4 weeks, while the sham group and model group were given water 10 ml/kg. Then their middle cerebral arteries were obstructed for 2 h and reperfused, excepted the sham group. 6 rats from each group were selected to test with Morris water maze 12 h, 24 h and 48 h after re-perfusion respectively. And then, their brain tissues were stained with Hematoxylin-Eosin staining to observe under optical microscope. The level of superoxide dismutase (SOD) and malondialdehyde (MDA) in the brain tissues were measured. Results Compared with the sham group, the latency significantly prolonged, and the incidence of crossing the area the platform located reduced in the model group in the Mor-ris water maze test, with the SOD decreasing and MDA increasing (P<0.05). Compared with the model group, the latency reduced and the incidence of crossing the area increased in the GSPE 200 mg/kg group, with the SOD increasing and MDA decreasing (P<0.05). Conclu-sion GSPE may suppress peroxidation after the cerebral ischemia/reperfusion to protect brain and learning and memory ability from injury.关键词
脑缺血/再灌注/葡萄籽原花青素/水迷宫/超氧化物歧化酶/丙二醛/大鼠Key words
cerebral ischemia/reperfusion/grape seed proanthocyanidin extract/Morris water maze/superoxide dismutase/malondial-dehyde/rats分类
医药卫生引用本文复制引用
王珠,赵雅宁,李建民..葡萄籽原花青素对脑缺血再灌注大鼠氧化应激与学习记忆的影响[J].中国康复理论与实践,2014,(9):827-830,4.
