中国康复理论与实践Issue(9)：831-834,4.DOI:10.3969/j.issn.1006-9771.2014.09.010
电针对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响
Effect of Electroacupuncture on Learning and Memory Ability of Cerebral Ischemia/Reperfusion Injured Rats
摘要
Abstract
Objective To investigate the effect of electroacupuncture at Shenting (DU24) and Baihui (DU20) on learning and memory ability, as well as the nerve cell and the expression of mRNA of Bcl-2 and Bax in the hippocampus of rats with cerebral ischemia/reperfu-sion (I/R) injury. Methods 45 male Sprague-Dawley rats were randomly diveded into sham group (n=15), control group (n=15) and elec-troacupuncture group (n=15). The latter 2 groups were modeled with middle cerebral artery occlusion for 2 h and reperfusion. The elec-troacupuncture group received electroacupuncture at Shenting and Baihui for 7 days. They were tested with Morris water maze, and then, their hippocampus was observed under Nissl staining and the level of Bcl-2 and Bax mRNA were determined with RT-PCR. Results The es-cape latency decreased (P<0.001) and the frequence the platform was crossed increased (P=0.001) in the electroacupuncture group com-pared with the model group, with less damage of the nerve cell and the expression of Bax mRNA, and more of Bcl-2 mRNA (P<0.05). Con-clusion Electroacupuncture can prevent learning and memory ability from cerebral ischemia/reperfusion injury in rats, which may associate with adjustment of expression of apoptosis-related gene to inhibit apoptosis of nerve cell.关键词
脑缺血/再灌注/电针/学习记忆/凋亡/Bax/Bcl-2Key words
cerebral ischemia/reperfusion/electroacupuncture/learning and memory/apoptosis/Bax/Bcl-2分类
医药卫生引用本文复制引用
林浴坤,陶静,陈斌,林如辉..电针对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响[J].中国康复理论与实践,2014,(9):831-834,4.基金项目
协同创新中西医结合基础研究实验专项(No.X2012004-协同)。 （No.X2012004-协同）
