中国康复理论与实践Issue(11)：1028-1030,3.DOI:10.3969/j.issn.1006-9771.2014.11.008
远志皂苷元对新生大鼠海马神经干细胞分化的影响
Effect of Tenuigenin on Differentiation of Neural Stem Cells from Hippocampus of Newborn Rats
摘要
Abstract
Objective To observe the effect of tenuigenin on differentiation of hippocampal neural stem cells (NSCs). Methods NSCs isolated from newborn (within 24 h) Wistar rats hippocampus were cultivated in vitro with serum free and clone culturing technology. Tenui-genin of different doses (0, 1, 2, 4μg/ml) were added in the medium, and the proliferation and differentiation of the cells were identified with immunofluorescence staining. Results The neural spheres obtained from the hippocampi of newborn rats were positive for Nestin ex-pression, with the potential for further cloning and differentiation into neurons or glial cells. The incidence of neuron specific enolase and glial fibrillary acidic protein positive cells increased in all the tenuigenin groups compared to the control (P<0.05), while the Nestin positive cells decreased (P<0.05). Conclusion Tenuigenin may promote the differentiation of neural stem cell into nerve cell.关键词
远志皂苷元/神经干细胞/细胞培养/细胞分化/大鼠Key words
tenuigenin/neural stem cells/cell culturing/cell differentiation/rats分类
医药卫生引用本文复制引用
陈玉静,黄小波,陈文强,王宁群..远志皂苷元对新生大鼠海马神经干细胞分化的影响[J].中国康复理论与实践,2014,(11):1028-1030,3.基金项目
国家自然科学基金项目(No.81102624)。 （No.81102624）
