中国康复理论与实践Issue(4)：382-386,5.DOI:10.3969/j.issn.1006-9771.2015.04.003
脊髓切开术对大鼠脊髓损伤区自噬的影响
Effects of Myelotomy on Autophagy in Injured Spinal Cord in Rats
摘要
Abstract
Objective To observe the effects of myelotomy on autophagy activation after traumatic spinal cord injury (SCI) in rats. Methods 54 adult female Sprague-Dawley rats were randomly assigned to sham-operated group (SG, n=18), contusion group (CG, n=18) or myelotomy group (MTG, n=18). The T10 SCI model in rats was induced with a New York University (NYU) impactor and myelotomy was performed 24 hours after SCI. They were evaluated with the BBB score 1, 7, 14 days after injury. The expression of mRNA of Beclin-1 and Bcl-2 were detected with real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The formation of autophagosome was investigated under electronic microscope (EM) 3 days after injury. Results BBB score was more in the MTG than in the CG 7 and 14 days after injury (P<0.05), while the expression of Beclin-1 mRNA was less (P<0.05). The expression of Bcl-2 mRNA was more in the MTG than in the CG 3 and 7 days after injury (P<0.05). The expression of Beclin-1 mRNA was negatively correlated with BBB scores (P<0.05). The formation of autophagosome was less in the MTG than in the CG. Conclusion Myelotomy can improve the recovery of motor function in rats after acute traumatic SCI, which may associate with neuroprotection mediated by inhibition of autophagy through the Bcl-2 signaling pathway.关键词
脊髓损伤/自噬/Beclin-1/Bcl-2/脊髓切开术Key words
spinal cord injury/autophagy/Beclin-1/Bcl-2/myelotomy分类
医药卫生引用本文复制引用
杨德刚,吴海丰,李建军,杨明亮,杜良杰,胡安明,顾蕊,陈亮,褚宏宇,张宪娣..脊髓切开术对大鼠脊髓损伤区自噬的影响[J].中国康复理论与实践,2015,(4):382-386,5.基金项目
1.国家自然科学基金项目(No.81272164)；2.中央级公益性科研院所基本科研业务费专项资金项目(No.2011CZ-2)。 （No.81272164）
