中国康复理论与实践Issue(4)：406-411,6.DOI:10.3969/j.issn.1006-9771.2015.04.009
碱性成纤维细胞生长因子-壳聚糖载体诱导神经干细胞向神经元分化并形成突触的研究
Basic Fibroblast Growth Factor-Chitosan Carriers Induce Neural Stem Cells to Differentiate into Neurons and Form Synapses
摘要
Abstract
Objective To explore the effect of basic fibroblast growth factor (bFGF)-chitosan carriers on neural differentiation of neural stem cells (NSCs). Methods NSCs were isolated from spinal cord of a neonatal Wistar rat and cultured. Purity of cultured NSCs was identi-fied with Nestin immunofluorescent staining. The 10 mg/ml chitosan carriers, 20 ng/ml bFGF or 10 mg/ml bFGF-chitosan carriers were add-ed into medium of P3~P4 NSCs respectively. NSCs were observed with immunofluorescent staining: 3 days after incubation with Nestin andβ-tubulin III;7 days after incubation with microtubule-associated protein-2 (MAP2), glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP);and 14 days after incubation with synapsin-1 and MAP2. The electrophysiological activity of cells was detected with MED64. Results 3 days after incubation, all the NSCs differentiated into Nestin+/β-tubulin III+, and the length of neurofilament was the high-est in those co-cultured with bFGF-chitosan carriers. 7 days after incubation, NSCs differentiated into MAP2+, GFAP+and MBP+, and more NSCs differentiated into MAP2+with bFGF-chitosan carriers. 14 days after incubation, NSCs differentiated with bFGF-chitosan carriers ex-press synapsin-1+/MAP2+and showed electrophysiological activity. Conclusion bFGF-chitosan carriers can induce NSCs to differentiate in-to neuron with high percentage and the differentiated neurons can form synapses with electrophysiology activity.关键词
神经干细胞/碱性成纤维细胞生长因子/壳聚糖/神经分化/突触/神经元Key words
neural stem cells/basic fibroblast growth factor/chitosan/neural differentiation/synapses/neurons分类
医药卫生引用本文复制引用
王聪,杨朝阳,段红梅,李晓光..碱性成纤维细胞生长因子-壳聚糖载体诱导神经干细胞向神经元分化并形成突触的研究[J].中国康复理论与实践,2015,(4):406-411,6.基金项目
1.国家自然科学基金面上项目(No.31271037)；2.国家自然科学基金国际合作与交流项目(No.31320103903)。 （No.31271037）
