中国康复理论与实践Issue(4)：412-416,5.DOI:10.3969/j.issn.1006-9771.2015.04.010
丁苯酞对SH-SY5Y细胞凋亡的影响
Effect of Butylphthalide on Apoptosis of SH-SY5Y Cells
摘要
Abstract
Objective To explore the effects of butylphthalide on the apoptosis of SH-SY5Y cells induced by the 1-methyl-4-phenylpyri-dinium iodide (MPP+). Methods SH-SY5Y cells were cultured with butylphthalide in the dosage of 1 µmol/L, 10 µmol/L and 20 µmol/L for 2 hours, and with MPP+for 24 hours. The viability of cells was measured with trypan blue. The mitochondrial membrane potential was de-tected with Rhodamine 123. The content of P53 protein was detected with ELISA. The expression of P53 and cytochrome C (CytC) were de-tected with Western blotting. Results The viability of SH-SY5Y cells increased with the dosage of butylphthalide (P<0.05), while the mito-chondrial membrane potential and the expression of P53 and CytC decreased (P<0.05). Conclusion Butylphthalide can prevents the SH-SY5Y cells from the injury induced by MPP+, which may associate with the inhibition of apoptosis through the expression of P53 and CytC.关键词
帕金森病/丁苯酞/SH-SY5Y细胞/P53/细胞色素C/凋亡Key words
Parkinson's disease/butylphthalide/SH-SY5Y cells/P53/cytochrome C/apoptosis分类
医药卫生引用本文复制引用
赵素晨,吴庆文,程月发,陈娟,贾玉凤,孟奇..丁苯酞对SH-SY5Y细胞凋亡的影响[J].中国康复理论与实践,2015,(4):412-416,5.基金项目
河北联合大学博士科研启动基金项目(No.35647699)。 （No.35647699）
