中国康复理论与实践Issue(5)：514-518,5.DOI:10.3969/j.issn.1006-9771.2015.05.005
时钟基因启动子甲基化频率随增龄的组织特异性改变
Tissue-specific Changes of Clock DNA Promoter Methylation with Aging
摘要
Abstract
Objective To investigate the role of the clock gene promoter methylation in aging. Methods C57BL mice of 4-(young, n=9) and 20-(old, n=10) month-old were determined the promoter methylation level of clock genes (Per1/2, Bmal1/2, Cry1/2, Clock, Npas2) in the stomach, spleen, vascular, kidney and striatum with methylation-specific polymerase chain reaction (MSP). Results The incidence of promoter methylation of Cry1, Bmal2 and Npas2 in spleen increased in old mice (P<0.05), while the promoter methylation of Per1 in stom-ach decreased (P<0.05), and the promoter methylation of Bmal1 in vascular increased (P<0.05). Conclusion Promoter methylation of some clock genes is involved in process of aging in a tissue-specific way.关键词
衰老/昼夜节律/时钟基因/启动子甲基化Key words
aging/circadian rhythm/clock genes/promoter methylation分类
医药卫生引用本文复制引用
祝艳秋,陆璐,李林,蔡彦宁,张兰..时钟基因启动子甲基化频率随增龄的组织特异性改变[J].中国康复理论与实践,2015,(5):514-518,5.基金项目
1.国家自然科学基金项目(No.81473373)；2.北京市自然科学基金项目(No.7132110)；3.北京市中医管理局重点项目(No. KJTS2011-04)；4.北京市新世纪百千万人才工程项目(No.008-0014)；5.首都卫生发展科研专项项目(No.首发2011-1001-05)；6.北京市教委新医药学科群项目(No.XK100270569)。 （No.81473373）
