摘要
Abstract
Objective: To build the beagle dog peri-implant bone defect model, and construct Gene Activated Matrix (GAM)containing plasmid pcDNA3.1 flag-Osx and mixed with autologous Bone Marrow Stromal Cells (BMSC);to evaluate the effect of Osx gene activation technology on bone formation and minerali-zation. Methods: With the marrow extracted prior to operation, bone marrow stromal cells were culti-vated and plasmid pcDNA3.1 flag-Osx amplified. Fifteen male beagle dogs were randomly whilst evenly divided into three groups, with group 1 being a four-week group, group 2 as an eight-week group and group 3 a twelve-week group. The left mandibular 2nd and 4th premolar and the rihgt mandibular 2nd premolar were extracted, based on which the operations of trapezoidal incisions and bone defects were made. Each of the three bone defects was implanted with an OSSTEM GSII, and filled respectively with:Category A, Perioglas;Category B, Perioglas+BMSCs+empty vector pcDNA3.1flag;and Category C, Perioglas+BMSCs+pcDNA3.1flag-Osx. Each material was covered with Bio-Gide collagen membrane via Guided Bone Regeneration (GBR) technique. Finally in 4, 8, and 12 weeks after the operation, ani-mals in group 1, 2 and 3 were killed respectively to obtain the specimens. The specimens were analyzed by comparison with the control group utilizing histological, imageological, and histomorphometric mea-surements. Each index is weighted by Mean ±Standard deviation (X ±s). The index differences between groups at each time sample are analyzed using the statistical package SPSS 17.0 by one way analysis of variance (ANOVA). P<0.05 were considered statistically significant. Results:1. Sclerous tissue exami-nation. Newly formed bone appeared at every bone defect under tests, among which Group C has the richest new bones, Group B has less and Group A has the least. 2.Results from Micro-CT analysis:Group 1: Except BVF, all the other indexes were significant different between category B and A (P<0.05). In terms of Trabecular Spacing (Tb.Sp), Trabecular Number (Tb.N), Bone Volume Fraction (BVF) and Bone Volume (BV), significant difference existed between category C and category B (P<0.05). For each index, there was significant difference between category C and A (P<0.05). Group 2: Except BVF, all the other indexes were significant different between category B and A (P<0.05). In terms of Tb.Sp, Tb.N, BV and Trabecular Thickness(Tb.Th), significant difference existed between category C and cate-gory B (P<0.05). For each index, there was significant difference between category C and A (P<0.05). Group 3: For each index, there was significant difference between category B and A (P<0.05). Except BVF, all the other indexes were significant different between category C and B ( P<0.05) . For each in-dex, there was significant difference between category C and A ( P<0.05) . Conclusions: Superior to the solutions without applying Osx plasmid, gene activated matrix combined with bone marrow stromal cells and Osx plasmid offer a faster bone issue promoting speed. These materials have significant bone induction effect which leads to more neoformative bone mass regenerated.关键词
基因活化基质/Osx/骨髓基质干细胞/Micro-CT/骨缺损/种植体/硬组织切片Key words
gene activated matrix/Osx/bone marrow stromal cells/micro-CT/bone defect/im-plant/hard tissue slicing分类
医药卫生
